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Transfection

Companies Transfection

IBA GmbH, Germany

IBA GmbH – Solutions For Life Sciences IBA GmbH, Germany, is dedicated to providing high quality product and service solutions for life science research in industry and academia. The comprehensive portfolio ranges from nucleic acid custom services to products and services for cloning, transfection, more

Miltenyi Biotec GmbH, Germany

Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Since 1989, we have developed innovative and reliable technologies for scientists and clinicians around the world. Our integrated portfolio of tools covers techniques of sample prepar more

News Transfection

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White papers Transfection

  • Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

    Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

  • Trichostatin A Treatment of Cells Transfected with FuGENE® HD Transfection Reagent

    Treatment of eukaryotic cells with trichostatin A (TSA) - a broad-spectrum inhibitor of histone deacetylation - is frequently used in studies of epigenetic regulation of gene expression. To be able to investigate the effects of TSA on promoter activity in reporter gene assays it is necessary to secure high transfection efficiency and low toxicity of the transfection reagent. In this study we tested transfection efficiency and toxicity of FuGENE® HD Transfection Reagent in experiments with hepatoma cell line HepG2. We also established a protocol for TSA treatment that guarantees high cell survival and sufficient TSA effect more

  • Dynamics of Kinases Involved in Cell Growth Using the xCELLigence System

    Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool for mediating the loss-of-function of specific mRNA molecules and preventing their translation into proteins. Numerous studies have helped to uncover novel gene functions in many biological processes, but most have used single endpoint analysis as readout for the respective phenotypes to characterize the outcome of a cascade of regulatory mechanisms. The xCELLigence System offers a new way to analyze the effect of gene knock downs on living cells, as the system provides continuous, quantitative information about the electrical impedance at the bottom surface of microtiter plates. Thus, any change in cell number, cell morphology or cell attachment can be detected in real-time. more

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Publications Transfection

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Market studies Transfection

  • Stemgent, Inc. - Strategic SWOT Analysis Review

    Stemgent, Inc. - Strategic SWOT Analysis Review Summary Stemgent, Inc. (Stemgent) is a stem cell research company which works in sync with the world’s leading stem cell scientist to develop stem cell-qualified reagents including the small molecules, media, and tools for the generation of iPS cells more

Websites Transfection

Cell Projects: Electroporation, PCR, HTS, Isohelix DNA Products

Highly innovative Molecular Biology Solutions for Electroporation, PCR, DNA Buccal Swabs, DNA Isolation Kits and other Life Science Technologies. The Cell Projects ™ product division supplies quality plastic and chemical enabling kits for: Electroporation Transfection PCR HTS Isohelix DNA Isolation more

amsbio (AMS Biotechnology) - Your source for life science products and services. Heparanase, Zymolya

AMS Biotechnology (Europe) Ltd Your source for life science products and services. We have over 298,000 products in our catalogue. ISO 9001:2008 reg. Please Select.. By Keyword By Product Code Find an Antibody Find an Assay Kit Find a DNA Find a Protein Find a RNA By Accession Number By Clone Number more

ibidi - cells in focus

FREE SAMPLE PROGRAM Choose up to 3 different Slides for free!    >> more        MEET US AT: July 29, 2010 | Boston, MA LSE Show July 30, 2010 | Cambridge, MA LSE Show Aug 24-26 | Troy, USA ECIS User Meeting >> more IBIDI NEWS LifeAct-Plasmids Novel probe for visualization of Actin cytoskeleton >> mo more

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Definition

Transfection

Transfection Transfection describes the introduction of foreign material into eukaryotic cells using a virus vector or other means of transfer. The term transfection for non-viral methods is most often used in reference to mammal ian cells, whi ... more

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