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Ex Vivo Therapeutics: CellGro® Cell Processing Tools

High quality cell processing tools in GMP compliance for your clinical cell therapy: Cytokines, Media and Bioprocessing container more

News Serum-Free Media

• CellGenix Technologie Transfer GmbH: 15 Years of Innovation and Leadership

  2009 is a special year for CellGenix, according to the company. Cellgenix is celebrating its 15th anniversary. CellGenix was founded in May 1994 as a Spin-off from the University Medical Center Freiburg and has developed continuously ever since. 1997 the company launched the first GMP quality serum- more

• Millipore and Stem Cell Sciences To Provide Human Embryonic Stem Cell Media

  Millipore Corporation and Stem Cell Sciences (SCS) announced an exclusive license for Millipore to develop and market SCS' serum free media for the growth of human embryonic stem cells (hESCs). According to the company, the defined media will be the first product available in the industry that offer more

White papers Serum-Free Media

• Fugene® HD Transfection Reagent: Superior Performance for Challenging Expression Studies
  

  The ability to efficiently introduce foreign molecules into cells constitutes an important tool in any laboratory and facilitates studies of gene regulation and protein function. A plethora of transfection methods have been developed for transfer of DNA into mammalian and animal cells. Those methods either utilize the physicochemical properties of the vector-carrier of the DNA or use mammalian viruses. In the first group of methods, some classic techniques are well-known, such as: calcium phosphate, DEAE-dextran, electroporation liposome-mediated delivery, direct microinjection/biolistic particle delivery, and non-liposomal multi-component reagents. Viral vector systems use both DNA (Adeno-) and RNA (Retro-, Lenti-) viruses. more

• Optimized Production of Lentivirus Using FuGENE® HD Transfection Reagent
  

  Lentiviral vectors are ideally suited vehicles for a broad range of research applications. Depending on pseudotype, lentiviruses infect a wide range of cell types, non-discriminately transducing both dividing and non-dividing cells. As opposed to other popular vector delivery systems, lentivirus stably and rapidly integrates genetic payload into the host genome allowing for long-term studies in vivo. Current lentiviral vector systems can accommodate upwards of ten kilobases of foreign DNA [1], although promoter and enhancer elements reduce the practical size of gene open-reading frames to perhaps five or six kilo­bases, which is sufficient to accommodate most genes commonly studied in the mammalian genome. more

Publications Serum-Free Media

• Mass Spectrometry (LC–MS/MS) Identified Proteomic Biosignatures of Breast Cancer in Proximal Fluid

  We have begun an early phase of biomarker discovery in three clinically important types of breast cancer using a panel of human cell lines: HER2 positive, hormone receptor positive and HER2 negative, and triple negative (HER2-, ER-, PR-). We identified and characterized the most abundant secreted, s more

• Generation and clonal isolation of retinal stem cells from human embryonic stem cells

  Abstract Retinal stem cells (RSCs) are present within the pigmented ciliary epithelium (CE) of the adult human eye and produce progeny that differentiate in vitro into all neural retinal subtypes and retinal pigmented epithelium (RPE). We hypothesized that a RSC population, similar to the adult C more

• Generation of rat-induced pluripotent stem cells: Reprogramming and culture medium

  The rat represents an animal model highly attractive for studying pharmacology, physiology, aging, cardiovascular diseases, etc., that in many aspects is more adequate than the mouse model. Derivation of induced pluripotent stem cells from rats (riPS) opens the opportunity for gene targeting in spec more

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