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Quality for PCR: Highly-active Taq polymerase and mastermixes

PCR reagents for standard use and special PCR applications. Highly-active, robust PCR reagents for routine use more

Unique RNAi tools for powerful gene silencing in vitro and in vivo

Our pre-designed, customized or validated RNAi tools and services are dedicated to help life scientist achieve highly efficient gene silencing more

News Nucleotides

• Electronic zippers control DNA strands

  The DNA double helix has been one of the most recognisable structures in science ever since it was first described by Watson and Crick almost 60 years ago (paper published in Nature in 25 April 1953). The binding and unbinding mechanism of DNA strands is vital to natural biological processes and to more

• New insights into the signaling network of the vital protein mTOR
  

  Many diseases are caused by malfunction of the mTOR signaling network. Accurate knowledge of network protagonists could therefore provide new therapeutic targets. The research group of Prof. Michael N. Hall at the Biozentrum of the University of Basel has now identified a number of new mTOR-regulate more

• For unzipping DNA mysteries - literally - Cornell physicists discover how a vital enzyme works

  With an eye toward understanding DNA replication, Cornell researchers have learned how a helicase enzyme works to actually unzip the two strands of DNA (Nature, 2011).At the heart of many metabolic processes, including DNA replication, are enzymes called helicases. Acting like motors, these proteins more

White papers Nucleotides

• RealTime ready - Function Tested qPCR Assays Based on the Universal ProbeLibrary Technology
  

  RealTime ready qPCR Assays redefine real-time qPCR because each assay is function-tested in PCR and qualified under strict criteria to assure superior performance. The RealTime ready Assays are based on the well-known Universal ProbeLibrary (UPL) technology and are available in a ready-to-use formulation as dried-down assays in LightCycler® 480 Multiwell Plates 96 and 384, or as a primer probe mix for single assays in a liquid formulation for all real-time PCR platforms available in the market more

• Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology
  

  MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA transcripts annotated. Although miRNAs represent a relatively abundant class of transcripts, their expression levels vary greatly among species and tissues. Less abundant miRNAs routinely fail detection using technologies such as cloning, northern hybridization and microarray analysis. Here, we present a real-time quantification method for accurate, sensitive, and economical detection of plant miRNAs based upon the stem-loop RT primer approach using an miRNA-specific primer coupled with a stem-loop specific Universal ProbeLibrary (UPL) probe. This approach represents a highly specific, sensitive, and easy-to-apply system to detect miRNAs. more

• Rapid Screening by Direct Colony PCR Using the FastStart PCR Master
  

  The FastStart PCR Master is a convenient and ideal tool for direct colony PCR. Intact bacteria can be analyzed directly without prior template purification, FastStart PCR Master is therefore the tool of choice for rapid screening of cloning experiments. In this paper, we describe the use of the FastStart PCR Master for the amplification and cloning of mRNA including direct colony screening. more

Publications Nucleotides

• Mass spectrometry reveals synergistic effects of nucleotides, lipids, and drugs binding to a multidrug resistance efflux pump [Chemistry]

  Multidrug resistance is a serious barrier to successful treatment of many human diseases, including cancer, wherein chemotherapeutics are exported from target cells by membrane-embedded pumps. The most prevalent of these pumps, the ATP-Binding Cassette transporter P-glycoprotein (P-gp), consists of more

• Capillary Electrophoresis‐Mass Spectrometry Reveals the Distribution of Carbon Metabolites during Nitrogen Starvation in Synechocystis sp. PCC 6803

  Summary Nitrogen availability is one of the most important factors for the survival of cyanobacteria. Previous studies on Synechocystis revealed a contradictory situation with regard to metabolism during nitrogen starvation; that is, glycogen accumulated even though the expressions of sugar cata more

• Prevalence of Multinucleotide Replacements in Evolution of Primates and Drosophila

  Evolution of sequences mostly involves independent changes at different sites. However, substitutions at neighboring sites may co-occur as multinucleotide replacement events (MNRs). Here, we compare noncoding sequences of several species of primates, and of three species of Drosophila fruit flies, more

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