Luciferase

Companies Luciferase

Berthold Detection Systems GmbH, Germany

Titertek-Berthold is a life science company which is based in the USA and in Germany. The enterprise builds measurement instruments and sample processing equipment for biological samples. Customers are in academic and government research, pharma and biotech companies, foodstuff, human and veterinary more

Products Luciferase

New ATP Test for the Industrial Microbiology

Merck has developed a new ATP Test, HY-LiTE® Plus for monitoring of microbiological activity in metal working fluids, paints, coatings and waste water treatment more

News Luciferase

• Promega and BASF develop alternative method on identifying allergenic substances

  Promega and BASF have jointly developed an alternative method to animal studies that can reliably detect the allergenic potential of substances. Using a newly developed cell line, the reaction of skin cells to allergenic substances can now be demonstrated in the test tube. Allergic skin reactions in more

• Horizon Discovery Announces Strategic Partnership with Promega to Develop Next-Generation Predictive Human Disease Models

  Horizon Discovery Ltd.  announced a strategic partnership agreement with Promega Corporation. The partnership centers on the delivery of endogenous gene reporting capabilities, in the form of Promega’s luciferase and HaloTag® reporter technologies, using Horizon’s proprietary homologous recombinatio more

• Photoactivable bioluminescent probe

  A group of scientists led by Bengang Xing, at Nanyang Technological University, Singapore, in collaboration with Zhen Cheng and colleagues at Stanford University Medical Centre, California, have developed a photoactivable bioluminescent probe for imaging luciferase activity in live cells. Firefl more

White papers Luciferase

• High-Performance shRNA-based Gene Knockdown Using FuGENE® HD Transfection Reagent
  

  The function of proteins can be studied in cultured cells using two distinctly different methodologies, both involving the transfection of DNA or RNA into cultured cells. The protein can be over-expressed in cells by transfecting the gene that codes for the protein, or the expression of the protein can be decreased by introduction of short segments of interfering RNA termed either siRNA (short) or miRNA (micro). In the second method, the term gene knockdown, knockout, or gene silencing is used to define the decrease in the protein of interest. X-tremeGENE siRNA Transfection Reagent has proven very effective for delivery of both siRNA and miRNA, resulting in high levels of knockdown. more

• A High-Throughput Experimental Tool for Studying Gene Pathways
  

  The post-genome era has lead to great advances in high-throughput genomics studies. Genomic approaches such as microarray expression analyses and Chromatin Immuno-Precipitation on Chip (ChIP-chip) transcription factor binding assays have yielded new discoveries and insights into genetic pathways. These technologies provide valuable observational data but do not identify the functional connections within networks or explain the mechanism of gene regulation. Reporter gene based experiments like functional promoter assays provide an important additional layer of data for understanding mechanisms of regulation in genetic networks. Efficient and reproducible transfection methods are a prerequisite for all of these assays involving transfections. more

• Efficient Transfection of VeroE6 Cells with DNA and RNA for Luciferase Reporter Assays Using FuGENE® HD Transfection Reagent
  

  The interferon (IFN) system of mammalian cells is the first line of defense against viral infections. Following infection, danger signals such as double-stranded RNA or single-stranded RNA with an unmodified 5´ triphosphate end (e.g., VSV-RNA) are recognized by membrane-bound Toll-like receptors and/or cytoplasmic receptors (MDA5, RIG-I). This initiates a signal cascade which leads to the production of type I interferons. By autocrine and paracrine stimulation via the IFN-a/b receptor the expression of antiviral proteins is induced in the infected cell as well as in the surrounding cells. more

Publications Luciferase

• Over-expression of Neuron-derived Orphan Receptor-1 (NOR-1) exacerbates neointimal hyperplasia after vascular injury

  We have previously shown that NOR-1 (NR4A3) modulates the proliferation and survival of vascular cells in culture. However, in genetically modified animal models, somewhat conflicting results have been reported concerning the involvement of NOR-1 in neointimal formation after vascular injury. The a more

• Putative tumor suppressor gene SEL1L was downregulated by aberrantly upregulated hsa‐mir‐155 in human pancreatic ductal adenocarcinoma

  Abstract Sel‐1‐like (SEL1L) is a putative tumor suppressor gene that is significantly downregulated in human pancreatic ductal adenocarcinoma (PDA). The mechanism of the downregulation is unclear. Here, we investigated whether aberrantly upregulated microRNAs (miRNAs) repressed the expression o more

• Association of Aggression With a Novel MicroRNA Binding Site Polymorphism in the Wolframin Gene

  Abstract Rare mutations in the WFS1 gene lead to Wolfram syndrome, a severe multisystem disorder with progressive neurodegeneration and diabetes mellitus causing life‐threatening complications and premature death. Only a few association studies using small clinical samples tested the possible e more

Websites Luciferase