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Fluorescence Markers

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dianova GmbH, Germany

We have been providing our research and routine customers premium antibodies, immunoassays and products for molecular biology since 1982. We take the time to carefully consider all your queries and are pleased to support you in selecting primary antibodies, secondary antibodies and detection system more

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  • How our nerves regulate insulin secretion

    The autonomic nervous system, which is the part of the nervous system beyond conscious control, plays an important role in the release of insulin from beta cells in the endocrine part of the pancreas. The process by which this occurs has been a mystery, since it is difficult to give detailed study t more

  • The 2011 Körber Prize goes to Stefan Hell

    Prof. Dr. Dr. h. c. Stefan Hell of the Max Planck Institute for Biophysical Chemistry in Göttingen is to receive the 2011 Körber European Science Prize endowed with 750,000 euros for his pioneering discoveries in the field of optics. Every year, the Körber Prize is awarded to an outstanding scientis more

  • Visualising DNA sequences

    A new, fast way to analyse DNA could be used to sequence the genomes of viruses and in the future help tackle genetic disorders such as schizophrenia and congenital heart defects. Robert Neely and colleagues have used a DNA methyltransferase enzyme to label the 5’-GCGC-3’ DNA sequences with a fluo more

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  • Confocal Detection of NBT/BCIP In Situ Hybridization

    Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP precipitation by alkaline phosphatase in WMISH samples and confocal detection by reflection microscopy. WMISH protocols can also be combined with fluorescent counterstainings such as DAPI, antibodies, GFP lines, and fluorescent in situ probes. Such double and triple labeling combined with confocal microscopy allows expression profiling at a cellular resolution in cell types or embryological structures of interest. Whole-mount reflection CLSM will thus greatly facilitate large-scale, cellular resolution expression profiling in vertebrate and invertebrate model organisms. more

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