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58 Current white paper about the topic genes

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Outstanding Microarray Experimental Results Using FuGENE® HD Transfection Reagent

03-Jun-2009

Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently any effects on endogenous gene expression are gener

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Characterizing Environments: Recent Discoveries Enabled by Metagenomic Analysis with the Genome Sequencer FLX System

08-May-2009

Metagenomics is the study of genomic content in a complex mixture of microorganisms. The two primary goals of this approach are to characterize the organisms present in a sample and identify what roles each organism has within a specific environment.

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miRNA Extraction and Profiling on FFPE Samples

08-Sep-2008

RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21-18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification. In the past years, more than 500 novel genes have been ide

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Optimized Production of Lentivirus Using FuGENE® HD Transfection Reagent

27-Aug-2008

Lentiviral vectors are ideally suited vehicles for a broad range of research applications. Depending on pseudotype, lentiviruses infect a wide range

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Transfection of Human Tooth-Specific Lineage Cells with FuGENE® HD Transfection Reagent

22-Aug-2008

Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-fo

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High-Throughput and Complex Gene Expression Validation Using the Universal ProbeLibrary and the LightCycler® 480 System

03-Jun-2008

During the past decade, a considerable number of transcriptome profiling analyses have been reported. High-throughput approaches such as tagging-based serial analysis of gene expression (SAGE) and hybridization-based microarray technologies have been

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High-Performance shRNA-based Gene Knockdown Using FuGENE® HD Transfection Reagent

29-May-2008

The function of proteins can be studied in cultured cells using two distinctly different methodologies, both involving the transfection of DNA or RNA into cultured cells. The protein can be over-expressed in cells by transfecting the gene that codes

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Aphidicolin-Mediated Replication Stress Induces Submicroscopic Tumor-Like Deletions in FHIT/FRA3B

26-May-2008

Common fragile sites (CFSs) are loci that are particularly susceptible to forming gaps and breaks on metaphase chromosomes after partial inhibition of DNA synthesis, for example induced by aphidicolin (APH). FRA3B, a fragile site lying within the FHI

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Identification of a New Arenavirus Using High-Throughput Sequencing

23-May-2008

Culture, PCR, serologic, and oligonucleotide microarray analyses for a wide range of infectious agents were negative in samples from three individuals who had received organ transplants from a single donor and died of a febrile illness 4-6 weeks afte

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qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway

19-May-2008

With the completion of the human genome sequence, the detailed understanding of gene functions by analyzing the structure, biological activities, and dynamics of the encoded proteins has become the next challenge. The technology of RNA interference (

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