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49 Current white paper about the topic genes

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High-Throughput and Complex Gene Expression Validation Using the Universal ProbeLibrary and the LightCycler® 480 System

03-Jun-2008

During the past decade, a considerable number of transcriptome profiling analyses have been reported. High-throughput approaches such as tagging-based serial analysis of gene expression (SAGE) and hybridization-based microarray technologies have been

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High-Performance shRNA-based Gene Knockdown Using FuGENE® HD Transfection Reagent

29-May-2008

The function of proteins can be studied in cultured cells using two distinctly different methodologies, both involving the transfection of DNA or RNA into cultured cells. The protein can be over-expressed in cells by transfecting the gene that codes

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Identification of a New Arenavirus Using High-Throughput Sequencing

23-May-2008

Culture, PCR, serologic, and oligonucleotide microarray analyses for a wide range of infectious agents were negative in samples from three individuals who had received organ transplants from a single donor and died of a febrile illness 4-6 weeks afte

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qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway

19-May-2008

With the completion of the human genome sequence, the detailed understanding of gene functions by analyzing the structure, biological activities, and dynamics of the encoded proteins has become the next challenge. The technology of RNA interference (

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Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System

07-May-2008

The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sens

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Methylation of the Arginine 2 in Histone H3 Controls Deposition of Lysine 4 Tri-Methylation

30-Apr-2008

In a recent study, Kirmizis et al. demonstrated that H3R2 is methylated not only in mammals but also in Saccharomyces cerevisiae. By using a specific H3R2me2a antibody in a ChIP-on-Chip analysis the authors determined the distribution of the methylat

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Evolutionary Link Between Maternal Behavior and Eusociality Is Supported by Wasp Gene Expression

28-Apr-2008

A defining feature of eusociality is the presence of workers that abstain from reproduction and care for their siblings (i.e., workers forage for food and feed the brood). It has been proposed that this behavior evolved from maternal care behavior an

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New: Multiplex Assays with Universal ProbeLibrary and Assay Design for Many More Organisms

Full Functionality of Universal ProbeLibrary Assay Design for Many More Organisms

20-Feb-2008

Since the availability of sound sequence data, organisms such as maize, rice, yeast, and zebrafish are gaining more and more importance in academic and industrial research, and the need for specific and quantitative real-time PCR assays for these org

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Confocal Detection of NBT/BCIP In Situ Hybridization

Samples by Reflection Microscopy

18-Feb-2008

Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP pre

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Transfering PCRs to HRM-assays on the LightCycler® 480 System - Examples for BRCA1

14-Feb-2008

High-resolution melting curve analysis (hrMCA) is an attractive technique to scan for unknown mutations in genes. To evaluate how easy or difficult it is to design hrMCA assays using the LightCycler® 480 Instrument, we selected 3 different fragments

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