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61 Current white paper about the topic genes
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Dynamics of Kinases Involved in Cell Growth Using the xCELLigence System
08-06-2009
Introduction Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool for mediating the loss-of-function of specific mRNA molecules and preventing their ...
Outstanding Microarray Experimental Results Using FuGENE® HD Transfection Reagent
03-06-2009
Introduction Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently any effects on endogenous gene expression are generally irrelevant, unless the ...
Characterizing Environments: Recent Discoveries Enabled by Metagenomic Analysis with the Genome Sequencer FLX System
08-05-2009
Introduction Metagenomics is the study of genomic content in a complex mixture of microorganisms. The two primary goals of this approach are to characterize the organisms present in a sample and identify what roles each organism has within a specific environment. Metagenomics samples are ...
miRNA Extraction and Profiling on FFPE Samples
08-09-2008
Introduction RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21–18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification [1]. In the past years, more than 500 novel genes have been identified in animals and ...
Optimized Production of Lentivirus Using FuGENE® HD Transfection Reagent
27-08-2008
Introduction Lentiviral vectors are ideally suited vehicles for a broad range of research applications. Depending on pseudotype, lentiviruses infect a wide range of cell types, non-discriminately transducing both dividing and non-dividing cells. As opposed to other popular vector delivery ...
Transfection of Human Tooth-Specific Lineage Cells with FuGENE® HD Transfection Reagent
22-08-2008
Introduction Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-forming odontoblasts. To study ...
High-Throughput and Complex Gene Expression Validation Using the Universal ProbeLibrary and the LightCycler® 480 System
03-06-2008
#img4453# Introduction During the past decade, a considerable number of transcriptome profiling analyses have been reported. High-throughput approaches such as tagging-based serial analysis of gene expression (SAGE) and hybridization-based microarray technologies have been widely performed to ...
High-Performance shRNA-based Gene Knockdown Using FuGENE® HD Transfection Reagent
29-05-2008
Introduction The function of proteins can be studied in cultured cells using two distinctly different methodologies, both involving the transfection of DNA or RNA into cultured cells. The protein can be over-expressed in cells by transfecting the gene that codes for the protein, or the ...
Aphidicolin-Mediated Replication Stress Induces Submicroscopic Tumor-Like Deletions in FHIT/FRA3B
26-05-2008
Introduction Common fragile sites (CFSs) are loci that are particularly susceptible to forming gaps and breaks on metaphase chromosomes after partial inhibition of DNA synthesis, for example induced by aphidicolin (APH). FRA3B, a fragile site lying within the FHIT tumor suppressor gene, is a ...
Identification of a New Arenavirus Using High-Throughput Sequencing
23-05-2008
Culture, PCR, serologic, and oligonucleotide microarray analyses for a wide range of infectious agents were negative in samples from three individuals who had received organ transplants from a single donor and died of a febrile illness 4–6 weeks after transplantation. Palacios et al. used ...
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