Prometheus, powered by nanoDSF, is the instrument of choice for the easy, rapid and precise analysis of protein stability and aggregation more
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Biosimilar profiling using nanoDSF: Thermal unfolding of the originator and 17 biosimilar candidates
Comparison of triplicate thermal unfolding signals from the F350/F330 ratio and the single wavelength at 350 nm
Quantitative similarity profiling of nanoDSF data: Comparison of the unfolding signals and corresponding first derivative traces of the best match (sample #11) and the worst match (sample #01)
N-linked glycan analysis of the reference product and a candidate
The development of biosimilars requires extensive physicochemical characterization of biosimilar candidate molecules, which should match the quality profile of the reference molecule (originator). Here we use a novel method of thermal unfolding profiling to rapidly screen a variety of Fc-fusion protein biosimilar candidates. The best-in-class precision of the NanoTemper Technologies Prometheus nanoDSF instrument allowed for the ranking of biosimilar candidates according to the comparability of their unfolding profiles to the reference molecule. The results were in excellent agreement with conventional screening methods, while dramatically reducing sample consumption and measurement times. Thus, nanoDSF (miniaturized differential scanning fluorimetry) is a new and powerful tool for rapid screening approaches in biosimilar development. It enables narrowing down the number of promising candidates in hours instead of days or weeks.
The assessment of thermal stability parameters of biologics is an integral part of biopharmaceutical research. The ever growing number of biologics in the development pipelines worldwide demands rapid and precise methods to quickly screen large sets of conditions in an easy and straightforw ... more
We have some great news! FOCUS and Statista have ranked the 500 fastest-growing companies in Germany and we made the list! They identified companies with exceptional growth rates between the years 2012 and 2015 as “Growth Champions 2017”. We’re proud to announce that NanoTemper Technologies ... more
NanoTemper Technologies – We care about your research At NanoTemper Technologies, we develop highly innovative instrument and software solutions for biomolecular analysis. Our global application centers ensure unparalleled customer care and support. We are passionate about achieving excel ... more