Prometheus, powered by nanoDSF, is the instrument of choice for the easy, rapid and precise analysis of protein stability and aggregation more
To use all functions of this page, please activate cookies in your browser.
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
Chemical denaturation curve of a mAb, analyzed by detecting changes in the fluorescence emission ratio using the Prometheus NT.Plex
Chemical denaturation of lysozyme at different pH values
Comparison of aggregation prediction by chemical denaturation and long-term stability measurements in formulations 1-5. (A) Chemical denaturation data (B) Determination of monomer contents (C) Turbidity measurements
Analysis of mAb stability by nanoDSF. Thermal unfolding curves of mAb1 in formulations F01-F05
Analysis of mAb stability by nanoDSF with backreflection aggregation detection
One of the most important parameters in the development of therapeutic biologics is their long-term stability. While after purification being seemingly stable in a variety of formulations, many antibodies display very slow aggregation kinetics over time. This gradual aggregation could thus far only be evaluated by monitoring monomer contents and aggregates over months or even years. Predictive methods are therefore urgently needed to speed up the development of biologics. Here we demonstrate that the Prometheus NT.Plex by NanoTemper Technologies can be used for predicting long-term stability in only a few hours. The approach uses a combination of thermal and chemical unfolding analysis in a high-throughput setting. We show that chemical denaturation is a tool which can determine folding enthalpies of monoclonal antibodies (mAbs) to predict their long-term stability in formulation screenings. Using the Prometheus NT.Plex nanoDSF instrument with aggregation detection optics, we screened 5 formulations at different mAb concentrations for their thermal and chemical stability. The obtained unfolding data correlates with long-term turbidity and monomer content over time, showing that the Prometheus NT.Plex can be used to rapidly predict the long-term stability of biologics within 1 day.
The assessment of thermal stability parameters of biologics is an integral part of biopharmaceutical research. The ever growing number of biologics in the development pipelines worldwide demands rapid and precise methods to quickly screen large sets of conditions in an easy and straightforw ... more
We have some great news! FOCUS and Statista have ranked the 500 fastest-growing companies in Germany and we made the list! They identified companies with exceptional growth rates between the years 2012 and 2015 as “Growth Champions 2017”. We’re proud to announce that NanoTemper Technologies ... more
NanoTemper Technologies – We care about your research At NanoTemper Technologies, we develop highly innovative instrument and software solutions for biomolecular analysis. Our global application centers ensure unparalleled customer care and support. We are passionate about achieving excel ... more