The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More information about Roche Innovatis products will appear in future issues of Biochemica.
The Cellavista System represents a fast and flexible image-based platform designed to combine the advantages of brightfield imaging with fluorescence capabilities in order to deliver results for a broad range of cellular applications. Its laser-based focusing technology allows for fast and reliable automated image acquisition from user-defined positions inside microplates and other small cell culture vessels. The images are archived, and the image analysis results are displayed in the form of spreadsheets, heat maps, time charts, histograms and scatterplots. The Cellavista System can be integrated into larger automated systems for unattended image acquisition and processing. Due to its highly flexible software and hardware design, the Cellavista System covers a broad range of research applications including, but not limited to:
- Cell colony growth and selection
- Cell confluence and cell number
- Quality control of cell distribution in plates
- Cellular screening assays (e.g. cytotoxicity, apoptosis, expression of cellular proteins)
- Wound healing assay
- Advanced tumor models (e.g. spheroids)
- Stem cell differentiation
Speeding up cell line development
One key factor for process development in cell biology is the generation of fast-growing cell lines that stably express the desired proteins at a consistently high level. The most critical steps in cell line development include:
-Single cell cloning and colony selection
-Optimization of transfection efficiency
The Cellavista System increases speed and control during all of these essential steps by visualizing, analyzing and documenting cellular growth (kinetics, confluence, cell size distribution, etc.) and protein expression of fluorescently labeled proteins.
Cedex Cell Culture Analyzers – Cedex, Cedex HiRes, Cedex Yeast, and Cedex XS Analyzer
The Cedex Analyzers product line provides automated and semi-automated solutions for cell culture analysis based on the Trypan Blue exclusion method for determination of cell concentration and viability. The image-based technology also provides information about morphological parameters such as diameter and compactness. The Cultivation Time Chart allows for the comparison of growth curves from different cultures and includes an automated calculation of specific growth rate and doubling time. The latest software is designed for optimal data management and contains features that allow for secure data handling and traceability of results. Images and measurement results are both automatically stored, enabling the user to document the analyses for later review.
The Cedex, Cedex HiRes, and Cedex Yeast Analyzers perform fully automated sample handling, staining, cell counting and graphical analysis of the results. They are proven to fit into the GMP process, comply with 21 CFR Part 11 requirements, and can be embedded in fully automated cell analysis cabinets with remote control software for integration into online sampling systems. The Cedex Analyzer delivers reliable image analysis results for applications involving average size cells (10-15 µm diameter), while the Cedex HiRes Analyzer has a higher resolution and magnification, allowing for the detection of cells with diameters as low as 2 µm. The Cedex HiRes Analyzer enables applications involving morphological analysis of cell cultures, such as monitoring of minute changes in cell morphology during baculovirus infection of insect cells (e.g., Sander L, Harrysson A (2007) Cytotechnology 54:35-48). The Cedex Yeast Analyzer is optimally adapted to yeast cells and uses the methylene blue dye exclusion method to determine viability.
The Cedex XS (“Extra Small”) is a compact, semi-automated, maintenance-free system that provides fast results while maintaining high image quality and results. The XS uses disposable slides that require just 5 µl of cell culture sample. One measurement with the Cedex XS takes less than 20 seconds and detects cells with diameters as small as 5 µm.
CASY® Systems – DT, TT, TTC
The CASY® Cell Counter + Analyzer System is compliant with ISO 13319, the internationally accepted method for cell counting. An advanced approach to digital pulse processing called “Pulse Area Analysis” is used to obtain information from signals created when a cell or a particle passes through the high precision measurement pore in a low voltage field. Pulse Area Analysis provides a unique, high-resolution recording of the cell size distribution and is the basis for the dye-free, non-invasive determination of cell viability. The method provides a clear-cut differentiation between cell debris, dead cells, viable cells and cell aggregates. Volume differences of a fraction of a femtoliter can be detected. Different measurement capillaries, in combination with cell-specific setups, allow for measurement of all types of mammalian cells, as well as bacteria, yeast, algae, parasites, pollen, and sperm.
Three different CASY® Cell Counter + Analyzer Systems are available to meet different needs. The CASY® Model DT (“Double Tower “) determines cell and particle concentration quickly and reliably. The CASY® Model TT (“Triple Tower “), in addition to determining concentration, automatically determines size distribution and aggregation level. The CASY® Model TTC (“Triple Tower Computer“) is a fast and easy-to-use cell analyzer system for “Total Quality Control” of cell cultures. It provides integrated data evaluation and complete documentation. The CASY® Model TTC system monitors cell viability, size, and aggregation and conforms to the full range of guidelines for GLP/GMP as well as 21 CFR Part 11.
For more information about the products, including technical details, please visit www.innovatis.com/products.
This article was originally published in Biochemica 3/2009, pages 21-22. ©Springer Medizin Verlag 2009