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523 Newest Publications about the topic size exclusion chromatography


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Crystallization and Preliminary X-ray Diffraction Analysis of CsaA from P. torridus: A Chaperone Rarely Found in Archaea

13-Jun-2017 | Sharma, Archana; K. Pal, Ravi; Goel, Manisha, Chemical Biology, 2017

Abstract: Background and Objective: CsaA is a molecular chaperone known to prevent aggregation of preproteins and also involved in the post-translational translocation of the proteins across the cytoplasmic membrane after correct folding. CsaA is known to be present in prokaryotes but is ...


Alzheimer's Disease-associated Aβ42 Peptide: Expression and Purification for NMR Structural Studies

13-Jun-2017 | Serra-Batiste, Montserrat; Garcia-Castellanos, Raquel; Ninot-Pedrosa, Marti; Serra-Vidal, Bernat; Simon Berrow, Nich ..., Chemical Biology, 2017

Abstract: Background: The aggregation of the amyloid-beta peptide (Aβ) in the brain is strongly associated with Alzheimer's disease (AD). However, the heterogeneous and transient nature of this process has prevented identification of the exact molecular form of Aβ responsible for the ...


Visible light-switched cytosol release of siRNA by amphiphilic fullerene derivative to enhance RNAi efficacy in vitro and in vivo

22-May-2017 | Author(s): Jing Wang, Lifei Xie, Tao Wang, Fengxin Wu, Jie Meng, Jian Liu, Haiyan Xu, Acta Biomaterialia, 2017

Publication date: Available online 13 May 2017 Source:Acta Biomaterialia Author(s): Jing Wang, Lifei Xie, Tao Wang, Fengxin Wu, Jie Meng, Jian Liu, Haiyan Xu Cationic macromolecules are attractive for use as small interfering RNA (siRNA) carriers due to their performance in ...


Extrafibrillar collagen demineralization-based chelate-and-rinse technique bridges the gap between wet and dry dentin bonding

22-May-2017 | Author(s): Sui Mai, Chin-Chuan Wei, Li-sha Gu, Fu-cong Tian, Dwayne D. Arola, Ji-hua Chen, Yang Jiao, ..., Acta Biomaterialia, 2017

Publication date: Available online 9 May 2017 Source:Acta Biomaterialia Author(s): Sui Mai, Chin-Chuan Wei, Li-sha Gu, Fu-cong Tian, Dwayne D. Arola, Ji-hua Chen, Yang Jiao, David H. Pashley, Li-na Niu, Franklin R. Tay Limitations associated with wet-bonding led to the ...


Marine Drugs, Vol. 15, Pages 140: Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells

18-May-2017 | Wu, Junwen ; Zhou, Xuefei ; Zhang, Min ; Yao, Yun ; Han, Juanjuan ; Liu, Kehai, Marine Drugs, 2017

In this study, the extraction conditions of the crude polysaccharide from Cereus sinensis were optimized by response surface methodology. The optimum extraction conditions were: a ratio of raw material to water volume of 1:80 (g/mL); an extraction temperature of 72 °C; and an extraction time of 3 ...


Characterization and quantitation of PVP content in a silicone hydrogel contact lens produced by dual‐phase polymerization processing

16-May-2017 | Andrew J. Hoteling, William F. Nichols, Patricia S. Harmon, Shawn M. Conlon, Ivan M. Nuñez, Joseph Hoff, Orlando M. ..., Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2017

Abstract Polyvinylpyrrolidone (PVP) has been incorporated over the years into numerous hydrogel contact lenses as both a primary matrix component and an internal wetting agent to increase lens wettability. In this study, complementary analytical techniques were used to characterize the PVP ...


Multi-stimuli-responsive biohybrid nanoparticles with cross-linked albumin coronae self-assembled by a polymer-protein biodynamer

07-May-2017 | Author(s): Lin Wang, Li Liu, Bingyang Dong, Hanying Zhao, Mingming Zhang, Wenjuan Chen, Yanhang Hong, Acta Biomaterialia, 2017

Publication date: May 2017 Source:Acta Biomaterialia, Volume 54 Author(s): Lin Wang, Li Liu, Bingyang Dong, Hanying Zhao, Mingming Zhang, Wenjuan Chen, Yanhang Hong A thermoresponsive polymer-protein biodynamer was prepared via the bioconjugation of an aliphatic ...


Exploring Tyrosine‐Triazolinedione (TAD) Reactions for the Selective Conjugation and Cross‐Linking of N‐Carboxyanhydride (NCA) Derived Synthetic Copolypeptides

29-Mar-2017 | Saltuk B. Hanay, Bas Ritzen, Dermot Brougham, Aylvin A. Dias, Andreas Heise, Macromolecular Bioscience, 2017

Highly efficient functionalization and cross‐linking of polypeptides is achieved via tyrosine‐triazolinedione (TAD) conjugation chemistry. The feasibility of the reaction is demonstrated by the reaction of 4‐phenyl‐1,2,4‐triazoline‐3,5‐dione (PTAD) with tyrosine containing block copolymer ...


Physicochemical Characterization of the Shell Composition of PBCA‐Based Polymeric Microbubbles

29-Mar-2017 | Lia Appold, Yang Shi, Stephan Rütten, Alexander Kühne, Andrij Pich, Fabian Kiessling, Twan Lammers, Macromolecular Bioscience, 2017

Microbubbles (MB) are routinely used as contrast agents for ultrasound (US) imaging. In recent years, MB have also attracted interest as drug delivery systems. Soft‐shelled lipidic MB tend to be more advantageous for US imaging, while hard‐shelled polymeric MB appear to be more suitable for drug ...


In situ analysis of Bacillus licheniformis biofilms: Amyloid‐like polymers and eDNA are involved in the adherence and aggregation of the extracellular matrix

18-Feb-2017 | I. Randrianjatovo‐Gbalou, P. Rouquette, D. Lefebvre, E. Girbal‐Neuhauser, C‐E Marcato‐Romain, Journal of Applied Microbiology, 2017

Abstract Aims This study attempts to determine which exopolymeric substances are involved in the adherence and aggregation of a Bacillus licheniformis biofilm. Methods and Results The involvement of extracellular proteins and eDNA were particularly investigated using DNase and ...


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