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523 Newest Publications about the topic size exclusion chromatography

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Nitrilotriacetic Amine-Functionalized Polymeric Core–Shell Nanoparticles as Enzyme Immobilization Supports

15-Aug-2017 | Dominic Keller; Ana Beloqui; Mónica Martínez-Martínez; Manuel Ferrer; Guillaume Delaittre, Biomacromolecules, 2017

Nitrilotriacetic amine (NTA)-functionalized nanoparticles obtained by aqueous polymerization-induced self-assembly (PISA) are introduced as immobilization supports for polyhistidine-functionalized (His-tagged) enzymes. A novel initiator for nitroxide-mediated polymerization based on the nitroxide ...

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Metagenomic alkaline protease from mangrove sediment

14-Aug-2017 | Tharcilla B. A. Pessoa, Rachel P. Rezende, Eric de Lima Silva Marques, Carlos P. Pirovani, Thalis F. dos Santos, Ana ..., Journal of Basic Microbiology, 2017

Functional screening of metagenomic libraries is an important tool for the discovery of new molecules. The metabolic diversity of microorganisms enables survival in harsh environments and is related to the production of enzymes. In this study, we identified a protease‐producing clone from a ...

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Metagenomic alkaline protease from mangrove sediment

14-Aug-2017 | Tharcilla B. A. Pessoa, Rachel P. Rezende, Eric de Lima Silva Marques, Carlos P. Pirovani, Thalis F. dos Santos, Ana ..., Journal of Basic Microbiology, 2017

Functional screening of metagenomic libraries is an important tool for the discovery of new molecules. The metabolic diversity of microorganisms enables survival in harsh environments and is related to the production of enzymes. In this study, we identified a protease‐producing clone from a ...

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Primum non nocere - the effects of sodium hypochlorite on dentin as used in endodontics

13-Aug-2017 | Author(s): Li-sha Gu, Xue-qing Huang, Brandon Griffin, Brian R. Bergeron, David H. Pashley, Li-na Niu, F ..., Acta Biomaterialia, 2017

Publication date: Available online 8 August 2017 Source:Acta Biomaterialia Author(s): Li-sha Gu, Xue-qing Huang, Brandon Griffin, Brian R. Bergeron, David H. Pashley, Li-na Niu, Franklin R. Tay The medical literature is replete with the maxim ‘primum non nocere’, cautioning ...

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Staphylococcus aureus single-stranded DNA-binding protein SsbA can bind but cannot stimulate PriA helicase

27-Jul-2017 | Yen-Hua Huang; Hong-Hsiang Guan; Chun-Jung Chen; Cheng-Yang Huang, PLoS ONE, 2017

by Yen-Hua Huang, Hong-Hsiang Guan, Chun-Jung Chen, Cheng-Yang Huang Single-stranded DNA-binding protein (SSB) and PriA helicase play important roles in bacterial DNA replication restart process. The mechanism by which PriA helicase is bound and stimulated by SSB in Escherichia coli (Ec) has ...

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Polyol structure influences enzymatic hydrolysis of bio‐based 2,5‐furandicarboxylic acid (FDCA) polyesters

21-Jul-2017 | Karolina Haernvall, Sabine Zitzenbacher, Hassan Amer, Michael Thomas Zumstein, Michael Sander, Kristopher McNeill, M ..., Biotechnology Journal, 2017

Polyesters of 2,5‐furan dicarboxylic acid (FDCA) have gained attention since they can be regarded as the bio‐based alternatives to the petroleum based polyesters of terephthalic acid. However, only little is known about the biodegradation and enzymatic hydrolysis of FDCA‐based polyesters. This ...

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Characterization of a Cathepsin D Protease from CHO Cell‐Free Medium and Mitigation of its Impact on the Stability of a Recombinant Therapeutic Protein

20-Jul-2017 | Amareth Lim, Brandon L. Doyle, Gerard M. Kelly, Angelia M. Reed‐Bogan, Lawrence H. Breen, Parviz A. Shamlou, Peter K ..., Biotechnology Progress, 2017

Abstract During purification process development of a recombinant therapeutic protein, an endoproteolytic activity endogenous to the Chinese hamster ovary (CHO) cells and leading to degradation at particular hydrophobic amino acid residues (e.g., Phe and Trp) was observed when processing at ...

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Marine Drugs, Vol. 15, Pages 223: Synthesis of Poly(norbornene-methylamine), a Biomimetic of Chitosan, by Ring-Opening Metathesis Polymerization (ROMP)

14-Jul-2017 | Li, Na ; Wang, Huanhuan ; Qu, Xiaosai ; Chen, Yu, Marine Drugs, 2017

ROMP is an effective method for preparing functional polymers due to its having characteristics of “living” polymerization and rapid development of catalysts. In the present work, poly(norbornene-methylamine), a mimic of chitosan, was synthesized via ROMP reaction. The amino-protected product, ...

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Marine Drugs, Vol. 15, Pages 220: Maitotoxin-4, a Novel MTX Analog Produced by Gambierdiscus excentricus

11-Jul-2017 | Pisapia, Francesco ; Sibat, Manoëlla ; Herrenknecht, Christine ; Lhaute, Korian ; Gaiani, Greta ; Ferron, Pierre-Jea ..., Marine Drugs, 2017

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, ...

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Marine Drugs, Vol. 15, Pages 180: Production of Chitin from Penaeus vannamei By-Products to Pilot Plant Scale Using a Combination of Enzymatic and Chemical Processes and Subsequent Optimization of the Chemical Production of Chitosan by Response Surface Methodology

16-Jun-2017 | Vázquez, José A.; Ramos, Patrícia ; Mirón, Jesús ; Valcarcel, Jesus ; Sotelo, Carmen G.; Pérez-Martín, Ricardo I., Marine Drugs, 2017

The waste generated from shrimp processing contains valuable materials such as protein, carotenoids, and chitin. The present study describes a process at pilot plant scale to recover chitin from the cephalothorax of Penaeus vannamei using mild conditions. The application of a sequential ...

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