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29 Newest Publications about the topic secondary antibodies

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Direct Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

01-Jul-2017 | Kohl, T. O., Ascoli, C. A., CSH Protocols, 2017

The competitive enzyme-linked immunosorbent assay (ELISA) (cELISA; also called an inhibition ELISA) is designed so that purified antigen competes with antigen in the test sample for binding to an antibody that has been immobilized in microtiter plate wells. The same concept works if the ...

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Dual display of proteins on the yeast cell surface simplifies quantification of binding interactions and enzymatic bioconjugation reactions

16-Mar-2017 | Sungwon Lim, Jeff E. Glasgow, Maria Filsinger Interrante, Erica M. Storm, Jennifer R. Cochran, Biotechnology Journal, 2017

Abstract Yeast surface display, a well‐established technology for protein analysis and engineering, involves expressing a protein of interest as a genetic fusion to either the N‐ or C‐terminus of the yeast Aga2p mating protein. Historically, yeast‐displayed protein variants are flanked by ...

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Immunolocalization of Proteins in Fission Yeast by Electron Microscopy

01-Jan-2017 | Morphew, M. K., Giddings, T. H., McIntosh, J. R., CSH Protocols, 2017

Electron microscopy (EM) immunolocalization of antigens in fission yeast can be accomplished with cells processed by rapid freezing and freeze-substitution followed by embedding in acrylic or methacrylate resins. Microtome sections of embedded cells are collected onto EM grids. Primary antibodies ...

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A Microplate-Based Nonradioactive Protein Synthesis Assay: Application to TRAIL Sensitization by Protein Synthesis Inhibitors

21-Oct-2016 | Curtis J. Henrich, PLoS ONE, 2016

by Curtis J. Henrich Non-radioactive assays based on incorporation of puromycin into newly synthesized proteins and subsequent detection using anti-puromycin antibodies have been previously reported and well-validated. To develop a moderate- to high-throughput assay, an adaptation is here ...

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Evaluation of Quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays

04-Feb-2016 | Aarti Jain, Omid Taghavian, Derek Vallejo, Emmanuel Dotsey, Dan Schwartz, Florian G. Bell, Chad Greef, D. Huw Davies ..., PROTEOMICS, 2016

Organic fluorescent dyes are widely used for the visualization of bound antibody in a variety of immunofluorescence assays. However, the detection equipment is often expensive, fragile and hard to deploy widely. Quantum dots (Qdot®) are nanocrystals made of semiconductor materials that emit ...

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Multispot, label-free biodetection at a phantom plastic-water interface [Biophysics and Computational Biology]

04-Jun-2013 | Fabio Giavazzi; Matteo Salina; Roberto Cerbino; Mattia Bassi; Davide Prosperi; Erica Ceccarello; Francesco Damin; La ..., Proceedings of the National Academy of Sciences current issue, 2013

Recognizing and quantifying specific biomolecules in aqueous samples are constantly needed in research and diagnostic laboratories. As the typical detection procedures are rather lengthy and involve the use of labeled secondary antibodies or other agents to provide a signal, efforts have been ...

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Preparation of Drosophila Embryos for Quantitative Imaging of Gene Expression

01-Jun-2013 | Surkova, S., Myasnikova, E., Kozlov, K. N., Pisarev, A., Reinitz, J., Samsonova, M., CSH Protocols, 2013

Quantitative measurements derived using sophisticated microscopy techniques are essential for understanding the basic principles that control the behavior of biological systems. We have developed a five-step data pipeline to extract quantitative data on segmentation gene expression from confocal ...

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Visualizing Active Enzyme Complexes Using a Photoreactive Inhibitor for Proximity Ligation – Application on γ-Secretase

24-May-2013 | Sophia Schedin-Weiss et al., PLoS ONE, 2013

by Sophia Schedin-Weiss, Mitsuhiro Inoue, Yasuhiro Teranishi, Natsuko Goto Yamamoto, Helena Karlström, Bengt Winblad, Lars O. Tjernberg Here, we present a highly sensitive method to study protein-protein interactions and subcellular location selectively for active multicomponent enzymes. We ...

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Zebrafish AID is capable of deaminating methylated deoxycytidines

01-May-2013 | Hala Abdouni; Justin J. King; Mussa Suliman; Matthew Quinlan; Heather Fifield; Mani Larijani, Nucleic Acids Research, 2013

Activation-induced cytidine deaminase (AID) deaminates deoxycytidine (dC) to deoxyuracil (dU) at immunoglobulin loci in B lymphocytes to mediate secondary antibody diversification. Recently, AID has been proposed to also mediate epigenetic reprogramming by demethylating methylated cytidines (mC) ...

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Cholesterol Loading and Ultrastable Protein Interactions Determine the Level of Tumor Marker Required for Optimal Isolation of Cancer Cells

01-Apr-2013 | Jayati Jain; Gianluca Veggiani; Mark Howarth, Cancer Research, 2013

Cell isolation via antibody-targeted magnetic beads is a powerful tool for research and clinical applications, most recently for isolating circulating tumor cells (CTC). Nonetheless fundamental features of the cell–bead interface are still unknown. Here we apply a clinically relevant antibody ...

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