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2,321 Newest Publications about the topic purification

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Exosome purification based on PEG-coated Fe3O4 nanoparticles

22-Jun-2018 | Ming Chang; Yaw-Jen Chang; Pei Yu Chao; Qing Yu, PLoS ONE, 2018

by Ming Chang, Yaw-Jen Chang, Pei Yu Chao, Qing Yu Cancer cells secrete many exosomes, which facilitate metastasis and the later growth of cancer. For early cancer diagnosis, the detection of exosomes is a crucial step. Exosomes exist in biological fluid, such as blood, which contains various ...

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Viruses, Vol. 10, Pages 334: Biochemical and Functional Characterization of Mouse Mammary Tumor Virus Full-Length Pr77Gag Expressed in Prokaryotic and Eukaryotic Cells

18-Jun-2018 | Chameettachal, Akhil ; Pillai, Vineeta Narayana; Ali, Lizna Mohamed; Pitchai, Fathima Nuzra Nagoor; Ardah, Mustaf ..., Viruses, 2018

Viruses, Vol. 10, Pages 334: Biochemical and Functional Characterization of Mouse Mammary Tumor Virus Full-Length Pr77Gag Expressed in Prokaryotic and Eukaryotic Cells Viruses doi: 10.3390/v10060334 Authors: Akhil Chameettachal Vineeta Narayana Pillai Lizna Mohamed Ali Fathima Nuzra ...

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Evidence for a second regulatory binding site on PspF that is occupied by the C-terminal domain of PspA

15-Jun-2018 | Eyleen Sabine Heidrich; Thomas Brüser, PLoS ONE, 2018

by Eyleen Sabine Heidrich, Thomas Brüser PspA is a key component of the bacterial Psp membrane-stress response system. The biochemical and functional characterization of PspA is impeded by its oligomerization and aggregation properties. It was recently possible to solve the coiled coil ...

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Energies, Vol. 11, Pages 1551: Increasing Profits in Food Waste Biorefinery—A Techno-Economic Analysis

13-Jun-2018 | Bastidas-Oyanedel, Juan-Rodrigo ; Schmidt, Jens Ejbye, Energies, 2018

Energies, Vol. 11, Pages 1551: Increasing Profits in Food Waste Biorefinery—A Techno-Economic Analysis Energies doi: 10.3390/en11061551 Authors: Juan-Rodrigo Bastidas-Oyanedel Jens Schmidt The present manuscript highlights the economic profit increase when combining organic waste ...

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VirB8 homolog TraE from plasmid pKM101 forms a hexameric ring structure and interacts with the VirB6 homolog TraD [Biochemistry]

05-Jun-2018 | Bastien Casu; Charline Mary; Aleksandr Sverzhinsky; Aurélien Fouillen; Antonio Nanci; Christian Baron, Proceedings of the National Academy of Sciences current issue, 2018

The overproduction and purification of membrane proteins are intrinsically difficult, making their analysis challenging. We purified the TraE membrane protein from a bacterial conjugation system that is involved in plasmid transfer. Our results suggest that this protein forms hexamers with a ...

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Ammonium Sulfate Fractionation of Antibodies

01-Jun-2018 | Fishman, J. B., Berg, E. A., CSH Protocols, 2018

Before the widespread availability and use of Protein A (rabbit) or Protein G (rodent) for the purification of IgG, the use of an ammonium sulfate "cut" was the standard method to isolate IgG and other serum proteins. The addition of ammonium sulfate reduces the effective solubility of proteins ...

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Preparation of Antibody Using Caprylic Acid

01-Jun-2018 | Fishman, J. B., Berg, E. A., CSH Protocols, 2018

Caprylic acid has been used to enrich IgG from serum, ascites, and cell culture supernatants by precipitating the non-IgG serum proteins. By precipitating all of the unwanted serum proteins rather than the antibodies, the tendency of antibodies to aggregate when precipitated is avoided. This ...

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Marine Drugs, Vol. 16, Pages 190: Optimum Production Conditions, Purification, Identification, and Antioxidant Activity of Violaxanthin from Microalga Eustigmatos cf. polyphem (Eustigmatophyceae)

01-Jun-2018 | Wang, Feifei ; Huang, Luodong ; Gao, Baoyan ; Zhang, Chengwu, Marine Drugs, 2018

Marine Drugs, Vol. 16, Pages 190: Optimum Production Conditions, Purification, Identification, and Antioxidant Activity of Violaxanthin from Microalga Eustigmatos cf. polyphem (Eustigmatophyceae) Marine Drugs doi: 10.3390/md16060190 Authors: Feifei Wang Luodong Huang Baoyan ...

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Energies, Vol. 11, Pages 1263: Fe2(SO4)3-Catalyzed Levulinic Acid Esterification: Production of Fuel Bioadditives

15-May-2018 | Martins, Fernanda Pereira; Rodrigues, Fabio Avila; Silva, Marcio Jose, Energies, 2018

Energies, Vol. 11, Pages 1263: Fe2(SO4)3-Catalyzed Levulinic Acid Esterification: Production of Fuel Bioadditives Energies doi: 10.3390/en11051263 Authors: Fernanda Martins Fabio Rodrigues Marcio Silva In this work, we developed a route to synthesize fuel bioadditives based on ...

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Energies, Vol. 11, Pages 1263: Fe2(SO4)3-Catalyzed Levulinic Acid Esterification: Production of Fuel Bioadditives

15-May-2018 | Martins, Fernanda Pereira; Rodrigues, Fabio Avila; Silva, Marcio Jose, Energies, 2018

Energies, Vol. 11, Pages 1263: Fe2(SO4)3-Catalyzed Levulinic Acid Esterification: Production of Fuel Bioadditives Energies doi: 10.3390/en11051263 Authors: Fernanda Martins Fabio Rodrigues Marcio Silva In this work, we developed a route to synthesize fuel bioadditives based on ...

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