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26 Newest Publications about the topic protein characterization


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Small laccase from streptomyces coelicolor—an ideal model protein/enzyme for undergraduate laboratory experience

23-Dec-2017 | Ryan Cook, Drew Hannon, Jonathan N. Southard, Sudipta Majumdar, Biochemistry and Molecular Biology Education, 2017

Abstract A one semester undergraduate biochemistry laboratory experience is described for an understanding of recombinant technology from gene cloning to protein characterization. An integrated experimental design includes three sequential modules: molecular cloning, protein expression and ...


Editor’s Highlight: Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS): A Web-Based Tool for Addressing the Challenges of Cross-Species Extrapolation of Chemical Toxicity

01-Oct-2016 | Carlie A. LaLone; Daniel L. Villeneuve; David Lyons; Henry W. Helgen; Serina L. Robinson; Joseph A. Swintek; Travis ..., Toxicological Sciences, 2016

Conservation of a molecular target across species can be used as a line-of-evidence to predict the likelihood of chemical susceptibility. The web-based Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS; application was developed to simplify, ...


A broad‐based study on hyphenating new ionization technologies with MS/MS for PTM's and tissue characterization

20-Apr-2016 | Darrell D. Marshall, Ellen D. Inutan, Beixi Wang, Chih‐Wei Liu, Shameemah Thawoos, James Wager‐Miller, Ken Mackie, S ..., PROTEOMICS, 2016

Matrix‐assisted ionization (MAI) is a newly discovered method for converting compounds from the solid phase to gas‐phase ions having charge states similar to electrospray ionization (ESI), but without the need for high energy sources such as lasers or high voltage. Laserspray ionization (LSI) is ...


New insights on the mitochondrial proteome plasticity in Parkinson's disease

08-Jan-2016 | Miguel Aroso, Rita Ferreira, Ana Freitas, Rui Vitorino, Maria Gomez‐Lazaro, PROTEOMICS, 2016

Parkinson's disease (PD) is one of the most common neurodegenerative diseases which relentless progression results in severe disability. Although PD aetiology is unknown, growing evidences point to the mitochondrial involvement in the pathobiology of this disorder. So, it seems imperative to ...


Assessing the Utility of Circular Dichroism and FTIR Spectroscopy in Monoclonal‐Antibody Comparability Studies

27-Oct-2015 | Jasper C. Lin, Zephania Kwong Glover, Alavattam Sreedhara, Journal of Pharmaceutical Sciences, 2015

Protein characterization is a necessary activity during development, technical transfers, and licensure. One important aspect of protein characterization is higher order structure assessment, which can be accomplished in a variety of ways. Circular dichroism (CD) and Fourier transform infrared ...


Electron transfer dissociation provides higher‐order structural information of native and partially unfolded protein complexes

17-Jun-2015 | Frederik Lermyte, Frank Sobott, PROTEOMICS, 2015

Top‐down sequencing approaches are becoming ever more popular for protein characterization, due to the ability to distinguish and characterize different protein isoforms. Under non‐denaturing conditions, electron transfer dissociation (ETD) can furthermore provide important information on the ...


Nanopores for proteins

01-Jan-2015 | Tal Nawy, Nature Methods, 2015

Nature Methods 12, 38 (2015). doi:10.1038/nmeth.3240 Author: Tal Nawy Nanopores hold promise for single-protein characterization.


Proteome sequencing goes deep

22-Dec-2014 | Author(s): Alicia L Richards , Anna E Merrill , Joshua J Coon, Current Opinion in Chemical Biology, 2014

Publication date: February 2015 Source:Current Opinion in Chemical Biology, Volume 24 Author(s): Alicia L Richards , Anna E Merrill , Joshua J Coon Advances in mass spectrometry (MS) have transformed the scope and impact of protein characterization efforts. Identifying hundreds of ...


Cationic β-Lactoglobulin Nanoparticles as a Bioavailability Enhancer: Protein Characterization and Particle Formation

03-Jul-2013 | Zi Teng; Ying Li; Yangchao Luo; Boce Zhang; Qin Wang, Biomacromolecules, 2013

Cationic β-lactoglobulin (CBLG) was developed as a bioavailability enhancer for poorly absorbed bioactives. At most 11 anionic amino acid residues of β-lactoglobulin (BLG) were substituted by ethylenediamine (EDA), resulting in a highly positive surface charge (zeta potential up to 39 mV at pH ...


Protein TAILS: when termini tell tales of proteolysis and function

18-Mar-2013 | Current Opinion in Chemical Biology, 2013

February 2013 Publication year: 2013Source:Current Opinion in Chemical Biology, Volume 17, Issue 1 Among the hundreds of posttranslational modifications, limited proteolysis, also known as processing, is special: It is irreversible, near ubiquitous, and by trimming peptide chains ...


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