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181 Newest Publications about the topic protein analysis

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Ambient surface mass spectrometry–ion mobility spectrometry of intact proteins

15-Apr-2018 | Author(s): Rian L Griffiths, Klaudia I Kocurek, Helen J Cooper, Current Opinion in Chemical Biology, 2018

Publication date: February 2018 Source:Current Opinion in Chemical Biology, Volume 42 Author(s): Rian L Griffiths, Klaudia I Kocurek, Helen J Cooper Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of ...

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A New Colorimetric Platform for Protein Detection Based on Recognition‐Induced Cascade of Polymeric Nanoparticles Disassembly

11-Jan-2018 | Fang Fang, Jin Liu, Yushu Li, Junjiao Yang, Jing Yang, Macromolecular Bioscience, 2018

Abstract Despite great progress, it is still of high interest to explore new homogeneous assays for simple, visual, and selective protein detection. Herein, one new colorimetric sensor has been developed for visual detection of protein by using polymeric micelles as a sensing scaffold and the ...

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Small laccase from streptomyces coelicolor—an ideal model protein/enzyme for undergraduate laboratory experience

23-Dec-2017 | Ryan Cook, Drew Hannon, Jonathan N. Southard, Sudipta Majumdar, Biochemistry and Molecular Biology Education, 2017

Abstract A one semester undergraduate biochemistry laboratory experience is described for an understanding of recombinant technology from gene cloning to protein characterization. An integrated experimental design includes three sequential modules: molecular cloning, protein expression and ...

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In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus

15-Dec-2017 | Sandra Alves Araújo; Alexandra Martins dos Santos Soares; Carolina Rocha Silva; Eduardo Bezerra Almeida Júnior; Cláu ..., PLoS ONE, 2017

by Sandra Alves Araújo, Alexandra Martins dos Santos Soares, Carolina Rocha Silva, Eduardo Bezerra Almeida Júnior, Cláudia Quintino Rocha, André Teixeira da Silva Ferreira, Jonas Perales, Livio M. Costa-Júnior Gastrointestinal nematodes are a significant concern for animal health and ...

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Evaluation and validation of commercial antibodies for the detection of Shb

01-Dec-2017 | Güliz Vanli; Alvaro Cuesta-Marban; Christian Widmann, PLoS ONE, 2017

by Güliz Vanli, Alvaro Cuesta-Marban, Christian Widmann Antibodies are among the most important tools for protein detection but, prior to their usage, proper validation of their appropriateness for given applications is required. The utility of an antibody depends on its sensitivity and ...

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ER stress and subsequent activated calpain play a pivotal role in skeletal muscle wasting after severe burn injury

13-Oct-2017 | Li Ma; Wanli Chu; Jiake Chai; Chuanan Shen; Dawei Li; Xiaoteng Wang, PLoS ONE, 2017

by Li Ma, Wanli Chu, Jiake Chai, Chuanan Shen, Dawei Li, Xiaoteng Wang Severe burns are typically followed by hypermetabolism characterized by significant muscle wasting, which causes considerable morbidity and mortality. The aim of the present study was to explore the underlying mechanisms of ...

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Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection [Biophysics and Computational Biology]

26-Sep-2017 | Clinton H. Hansen; Darren Yang; Mounir A. Koussa; Wesley P. Wong, Proceedings of the National Academy of Sciences current issue, 2017

Protein detection and quantification play critical roles in both basic research and clinical practice. Current detection platforms range from the widely used ELISA to more sophisticated, and more expensive, approaches such as digital ELISA. Despite advances, there remains a need for a method that ...

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Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection [Biophysics and Computational Biology]

26-Sep-2017 | Clinton H. Hansen; Darren Yang; Mounir A. Koussa; Wesley P. Wong, Proceedings of the National Academy of Sciences current issue, 2017

Protein detection and quantification play critical roles in both basic research and clinical practice. Current detection platforms range from the widely used ELISA to more sophisticated, and more expensive, approaches such as digital ELISA. Despite advances, there remains a need for a method that ...

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MicroRNA‐214‐3p: A Link between Autophagy and Endothelial Cell Dysfunction in Atherosclerosis

09-Sep-2017 | Jing Wang, Wei‐Na Wang, Shuang‐Bin Xu, Hong Wu, Bing Dai, Dong‐Dong Jian, Mei Yang, Yu‐Tao Wu, Qiang Feng, Jian‐Hua ..., Acta Physiologica, 2017

Abstract Aim Endothelial cell injury assumes a fundamental part in the pathogenesis of atherosclerosis, and endothelial cell autophagy has protective effects on the development of atherosclerosis, though the underlying molecular regulation mechanism is indistinct. This study is aimed to ...

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Optimization and Modeling of Quadrupole Orbitrap Parameters for Sensitive Analysis toward Single-Cell Proteomics

01-Sep-2017 | Bingyun Sun; Jessica Rae Kovatch; Albert Badiong; Nabyl Merbouh, Journal of Proteome Research, 2017

Single-cell proteomics represents a field of extremely sensitive proteomic analysis, owing to the minute amount of yet complex proteins in a single cell. Without amplification potential as of nucleic acids, single-cell mass spectrometry (MS) analysis demands special instrumentation running with ...

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