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605 Newest Publications about the topic oligonucleotides

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Pharmaceuticals, Vol. 11, Pages 62: Potential Inherent Stimulation of the Innate Immune System by Nucleic Acid Aptamers and Possible Corrective Approaches

23-Jun-2018 | Bruno, John G., Pharmaceuticals, 2018

Pharmaceuticals, Vol. 11, Pages 62: Potential Inherent Stimulation of the Innate Immune System by Nucleic Acid Aptamers and Possible Corrective Approaches Pharmaceuticals doi: 10.3390/ph11030062 Authors: John G. Bruno It is well known that unmethylated ...

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A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus [Medical Sciences]

19-Jun-2018 | Daniela Castanotto; Xiaowei Zhang; Jessica Alluin; Xizhe Zhang; Jacqueline Rüger; Brian Armstrong; John Rossi; Arthu ..., Proceedings of the National Academy of Sciences current issue, 2018

The deregulation of miRNA function is critical in the pathogenesis of cancer and other diseases. miRNAs and other noncoding RNAs (ncRNAs) tightly regulate gene expression, often in the cell nucleus. Heretofore, there has been no understanding that there exists a general shuttling mechanism that ...

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Reversal of siRNA-mediated gene silencing in vivo

14-May-2018 | Ivan Zlatev; Adam Castoreno; Christopher R Brown; June Qin; Scott Waldron; Mark K Schlegel; Rohan Degaonkar; Svetlan ..., Nature Biotechnology, 2018

Reversal of siRNA-mediated gene silencing in vivo Reversal of siRNA-mediated gene silencing in vivo, Published online: 14 May 2018; doi:10.1038/nbt.4136 Gene silencing by siRNAs in vivo is reversed using sequence-specific high-affinity oligonucleotides.

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Nanovesicle-mediated delivery of anticancer agents effectively induced cell death and regressed intrahepatic tumors in athymic mice

10-May-2018 | Joseph George; Irene K. Yan; Tushar Patel, Laboratory Investigation, 2018

Hepatocellular carcinoma is highly resistant to chemotherapy. Here we evaluated the use and efficacy of milk-derived nanovesicles (MNV) as an approach to improve delivery of anticancer agents into HCC cells and intrahepatic tumors. We developed a protocol for isolation of MNVs from skim milk ...

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In Vitro Mutagenesis Using Double-Stranded DNA Templates: Selection of Mutants with DpnI

01-Mar-2018 | Forloni, M., Liu, A. Y., Wajapeyee, N., CSH Protocols, 2018

In this protocol, two oligonucleotides are used to prime DNA synthesis by a high-fidelity polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and have the same starting and ending positions on opposite strands of the plasmid DNA. The entire ...

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Inhibiting miRNA Function by Antisense Oligonucleotides in Cultured Mammalian Cells

01-Feb-2018 | Li, C., Zamore, P. D., CSH Protocols, 2018

Methods for transfecting antisense oligonucleotides (ASOs) into mammalian cells for suppression of miRNA function are described in this protocol. The effects of ASOs on the miRNA are evaluated by measuring the protein level of the putative miRNA target or the activity of a reporter bearing a 3' ...

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Preparation of Antisense Oligonucleotides to Inhibit miRNA Function

01-Feb-2018 | Li, C., Zamore, P. D., CSH Protocols, 2018

This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation of 2'-O-methyl-modifications in ASOs enhances their potency and resistance to degradation, whereas 3'-terminal cholesterol-conjugation facilitates ...

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Inhibiting miRNA Function by Antisense Oligonucleotides in Drosophila S2 Cells

01-Feb-2018 | Li, C., Zamore, P. D., CSH Protocols, 2018

This protocol describes methods to transfect antisense oligonucleotides (ASOs) into Drosophila S2 cells for suppression of miRNA function. The effects of ASOs on the miRNA are evaluated by measuring the protein level of the putative miRNA target or the activity of a reporter bearing a 3' UTR ...

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Multiplexed gene synthesis in emulsions for exploring protein functional landscapes

19-Jan-2018 | Calin Plesa; Angus M. Sidore; Nathan B. Lubock; Di Zhang; Sriram Kosuri, Science , 2018

Improving our ability to construct and functionally characterize DNA sequences would broadly accelerate progress in biology. Here, we introduce DropSynth, a scalable, low-cost method to build thousands of defined gene-length constructs in a pooled (multiplexed) manner. DropSynth uses a library of ...

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Detection and identification of staphylococcus aureus using magnetic particle enhanced surface plasmon spectroscopy

08-Jan-2018 | Vicky Tröger, Nadine Rüppel, Natalia Sandetskaya, Dirk Kuhlmeier, Stefan Schmieder, Frank Sonntag, Engineering in Life Sciences, 2018

Abstract In this work an approach for surface plasmon resonance (SPR) spectroscopy using the liSPR system is examined that combines signal amplification by PCR and magnetic nanoparticles in one injection step. Therefore, the synthesis of PCR products was performed on the beads similar to a ...

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