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15 Newest Publications about the topic nucleic acid extraction


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Viruses, Vol. 9, Pages 63: Simultaneous Detection of Both RNA and DNA Viruses Infecting Dry Bean and Occurrence of Mixed Infections by BGYMV, BCMV and BCMNV in the Central-West Region of Mexico

30-Mar-2017 | Chiquito-Almanza, Elizabeth ; Acosta-Gallegos, Jorge A.; García-Álvarez, Nadia C.; Garrido-Ramírez, Eduardo R.; Mont ..., Viruses, 2017

A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed to simultaneously detect bean common mosaic virus (BCMV), bean common mosaic necrotic virus (BCMNV), and bean golden yellow mosaic virus (BGYMV) from common bean leaves dried with silica gel using a single ...


Viruses, Vol. 8, Pages 12: Quantifying Next Generation Sequencing Sample Pre-Processing Bias in HIV-1 Complete Genome Sequencing

07-Jan-2016 | Vrancken, Bram ; Trovão, Nídia Sequeira; Baele, Guy ; van Wijngaerden, Eric ; Vandamme, Anne-Mieke ; van Laethem, Kr ..., Viruses, 2016

Genetic analyses play a central role in infectious disease research. Massively parallelized “mechanical cloning” and sequencing technologies were quickly adopted by HIV researchers in order to broaden the understanding of the clinical importance of minor drug-resistant variants. These efforts ...


Prevalence of human papillomavirus infection in women in some cities and regions of Bulgaria

12-Jul-2013 | Stefan Kovachev, Victor Slavov, Kremena Slavova, Journal of Medical Virology, 2013

ABSTRACT This aim of this study was to evaluate the prevalence of human papillomavirus (HPV) infections among women (aged 15–55 years) in four of the biggest cities and regions in Bulgaria (viz., Sofia, Plovdiv, Varna, and Burgas), as well as in two other smaller cities (viz., Pleven and ...


Nucleic acid extraction methods from fixed and paraffin-embedded tissues in cancer diagnostics

09-Apr-2013 | Serena Bonin; Giorgio Stanta, Expert Review of Molecular Diagnostics, 2013

Diagnostic tests, based on nucleic acid extracts from formalin-fixed and paraffin-embedded tissues, are now becoming increasingly common due to the introduction of biological agents for cancer therapy. Unfortunately, the formalin-fixed and paraffin-embedded tissues are heterogeneous in terms of ...


Assessment of poly‐L‐Lysine dendrigrafts for virus concentration in water: use of MS2 bacteriophage as proof of concept

01-Apr-2013 | Axelle Cadiere, Benoit Couturaud, Jeanne Boismard, Pierre Cann, Anne Gérard, André Mas, Clement Faye, Laurent Garrel ..., Journal of Applied Microbiology, 2013

Abstract Aims Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly‐L‐Lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real ...


Cobas® 4800: a fully automated system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae

11-Mar-2013 | Barbara Van Der Pol, Expert Review of Molecular Diagnostics, 2013

Chlamydia trachomatis and Neisseria gonorrhoeae (CT/NG) are the most common sexually transmitted bacterial infections globally and account for the majority of the infections monitored by public health surveillance systems. Therefore, access to diagnostic tools that facilitate identification of ...


Deep Sequencing of RNA from Ancient Maize Kernels

11-Jan-2013 | Sarah L. Fordyce et al., PLoS ONE, 2013

by Sarah L. Fordyce, Maria C. Ávila-Arcos, Morten Rasmussen, Enrico Cappellini, J. Alberto Romero-Navarro, Nathan Wales, David E. Alquezar-Planas, Steven Penfield, Terence A. Brown, Jean-Philippe Vielle-Calzada, Rafael Montiel, Tina Jørgensen, Nancy Odegaard, Michael Jacobs, Bernardo Arriaza, ...


Development and application of a one‐step real‐time RT‐PCR using a minor‐groove‐binding probe for the detection of a novel bunyavirus in clinical specimens

04-Dec-2012 | Zhifeng Li, Lunbiao Cui, Minghao Zhou, Xian Qi, Changjun Bao, Jianli Hu, Jun Shan, Bin Wu, Shenjiao Wang, Xiling Guo ..., Journal of Medical Virology, 2012

Abstract A highly sensitive one‐step real‐time RT‐PCR method using a minor‐groove‐binding (MGB) probe was developed for detection and quantitation of severe febrile with thrombocytopenia syndrome virus (SFTSV). The assay could discriminate SFTSV infection from other related viral diseases in ...


Near instrument-free, simple molecular device for rapid detection of herpes simplex viruses

18-Jun-2012 | Bertrand Lemieux; Ying Li; Huimin Kong; Yi-Wei Tang, Expert Review of Molecular Diagnostics, 2012

The first near instrument-free, inexpensive and simple molecular diagnostic device (IsoAmp® HSV, BioHelix Corp., MA, USA) recently received US FDA clearance for use in the detection of herpes simplex viruses (HSV) in genital and oral lesion specimens. The IsoAmp HSV assay uses isothermal ...


Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens

01-Jun-2012 | Mengelle, C.; Mansuy, J.‐M.; Sandres‐Sauné, K.; Barthe, C.; Boineau, J.; Izopet, J., Journal of Medical Virology, 2012

Abstract The aim of the study was to evaluate the MagNA Pure 96™ nucleic acid extraction system using clinical respiratory specimens for identifying viruses by qualitative real‐time PCR assays. Three extraction methods were tested, that is, the MagNA Pure LC™, the COBAS Ampliprep™, and the ...


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