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17 Newest Publications about the topic hplc accessories

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Distribution of glucosinolates in camelina seed fractions by HPLC‐ESI‐MS/MS

03-May-2016 | Deyun Yuan, Youn Young Shim, Jianheng Shen, Pramodkumar D. Jadhav, Venkatesh Meda, Martin J. T. Reaney, European Journal of Lipid Science and Technology, 2016

Abstract High glucosinolate concentrations were detected and identified in camelina (Camelina sativa L. Crantz.) seed fractions using reversed phase high performance liquid chromatography‐electrospray ionization‐mass spectrometry (HPLC‐ESI‐MS) in multiple reaction monitoring mode. Total ...

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Development and validation of an LCMS method to determine the pharmacokinetic profiles of caffeic acid phenethyl amide and caffeic acid phenethyl ester in male Sprague–Dawley rats

24-Aug-2013 | John Yang, Phillip D. Bowman, Sean M. Kerwin, Salomon Stavchansky, Biomedical Chromatography, 2013

ABSTRACT A validated LCMS method was developed for the quantitative determination of caffeic acid phenethyl amide (CAPA) and caffeic acid phenethyl ester (CAPE) from rat plasma. Separation was achieved using a reverse‐phase C12 HPLC column (150 × 2.00 mm, 4 µm) with gradient elution running ...

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Asperterrestide A, a Cytotoxic Cyclic Tetrapeptide from the Marine-Derived Fungus Aspergillus terreus SCSGAF0162

12-Jun-2013 | Fei He; Jie Bao; Xiao-Yong Zhang; Zheng-Chao Tu; Yi-Ming Shi; Shu-Hua Qi, Journal of Natural Products, 2013

A new cytotoxic and antiviral cyclic tetrapeptide, asperterrestide A (1), a new alkaloid, terremide C (2), and a new aromatic butenolide, aspernolide E (3), together with 10 known compounds were isolated from the fermentation broth of the marine-derived fungus Aspergillus terreus SCSGAF0162. ...

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One set system for the synthesis and purification of glycosaminoglycan oligosaccharides reconstructed using a hyaluronidase‐immobilized column

10-Jun-2013 | Shinichiro Suto, Ikuko Kakizaki, Toshiya Nakamura, Masahiko Endo, Biopolymers, 2013

Abstract Using the transglycosylation reaction as a reverse reaction for the hydrolysis of hyaluronidase, new artificial oligosaccharides may be synthesized by reconstructing natural glycosaminoglycans according to preliminary planned arrangements. However, as some problems have been ...

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Measuring CD38 (ADP-Ribosyl Cyclase/Cyclic ADP-Ribose Hydrolase) Activity by Reverse-Phase HPLC

01-Jun-2013 | Kirchberger, T., Guse, A. H., CSH Protocols, 2013

Cyclic ADP-ribose (cADPR) is a Ca2+-mobilizing second messenger active in many cell types, tissues, and organisms. The mammalian NAD-glycohydrolase CD38 catalyzes formation of cADPR by removing nicotinamide and forming a new intramolecular bond between N1 of adenine and C1 of the "northern" ...

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Targeting human serum fucome by an integrated liquid‐phase multi column platform operating in “Cascade” to facilitate comparative mass spectrometric analysis of disease‐free and breast cancer sera

27-Mar-2013 | Subhashini Selvaraju, Ziad El Rassi, PROTEOMICS, 2013

A fully integrated platform was developed for capturing/fractionating human fucome from disease‐free and breast cancer sera. It comprised multicolumn operated by HPLC pumps and switching valves for the simultaneous depletion of high abundance proteins via affinity‐based subtraction and the ...

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Analysis of the Mushroom Nephrotoxin Orellanine and Its Glucosides

09-Oct-2012 | Anders Herrmann; Heidi Hedman; Johan Rosén; Daniel Jansson; Börje Haraldsson; Karl-Erik Hellenäs, Journal of Natural Products, 2012

Orellanine is a nephrotoxin found in various Cortinaceae mushroom species. Unintentional consumption after these species were confused with edible mushrooms such as Cantharellus tubaeformis has caused several casualties. In this work, a quantitative HPLC-ESI-MS/MS method for total orellanine in ...

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Synthesis of fatty acid based 3,6‐disubstituted‐1,2,3,6‐tetrahydro‐phthalic acid anhydride derivatives

20-Aug-2012 | Steven Eschig, Claudia Philipp, Tunga Salthammer, European Journal of Lipid Science and Technology, 2012

Abstract Fatty acid based 3,6‐disubstituted‐1,2,3,6‐tetrahydro‐phthalic acid anhydride derivatives were synthesized by Rhodium catalysed addition of maleic anhydride to linoleic and oleic acid. The maleinisation of linoleic acid leads to cyclohexenoic anhydrides having an allylic double bond. ...

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Antioxidant Properties of the Peptides Isolated From Ganoderma lucidum Fruiting Body

26-May-2012 | Vinay U. Girjal, Shivayogeeswar Neelagund, Madappa Krishnappa, International Journal of Peptide Research and Therapeutics, 2012

Ganoderma lucidum is widely used as traditional medicine for centuries particularly in China, Japan and Korea. Many bioactive metabolites isolated from G. lucidum were therapeutically active against various diseases. The peptide isolated from water extract of G. lucidum was purified by employing ...

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Direct 13C NMR Detection in HPLC Hyphenation Mode: Analysis of Ganoderma lucidum Terpenoids

19-Apr-2012 | Sileshi G. Wubshet; Kenneth T. Johansen; Nils T. Nyberg; Jerzy W. Jaroszewski, Journal of Natural Products, 2012

Solid phase extraction (SPE) was introduced as a crucial step in the HPLC-SPE-NMR technique to enable online analyte enrichment from which proton-detected NMR experiments on submicrogram amounts from complex mixtures were possible. However, the significance of direct-detected 13C NMR experiments ...

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