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365 Newest Publications about the topic high-throughput screening


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Novel statistical approach for evaluating flow cytometric in vitro micronucleus data

22-Aug-2016 | J.P. Wojciechowski, C.R. Gleason, D.J. Roberts, L.L. Custer, Environmental and Molecular Mutagenesis, 2016

Statistical methods currently recommended for analysis of in vitro micronucleus data are based on small sample sizes. The tests are designed to evaluate linear trends and differences between treated and control samples. When using flow cytometric analysis, >5 times the number of cells are easily ...


Comprehensive analysis of high-throughput screens with HiTSeekR

19-Aug-2016 | Markus List; Steffen Schmidt; Helle Christiansen; Marc Rehmsmeier; Qihua Tan; Jan Mollenhauer; Jan Baumbach, Nucleic Acids Research, 2016

High-throughput screening (HTS) is an indispensable tool for drug (target) discovery that currently lacks user-friendly software tools for the robust identification of putative hits from HTS experiments and for the interpretation of these findings in the context of systems biology. We developed ...


High throughput screening of disulfide‐containing proteins in a complex mixture

09-Sep-2013 | Dong S. Zhao, Zachery R. Gregorich, Ying Ge, PROTEOMICS, 2013

Abstract The formation of disulfide bonds between cysteine residues is crucial for the stabilization of native protein structures and, thus, determination of disulfide linkages is an important facet of protein structural characterization. Nonetheless, the identification of disulfide bond ...


AC1MMYR2, an Inhibitor of Dicer-Mediated Biogenesis of Oncomir miR-21, Reverses Epithelial–Mesenchymal Transition and Suppresses Tumor Growth and Progression

01-Sep-2013 | Zhendong Shi; Junxia Zhang; Xiaomin Qian; Lei Han; Kailiang Zhang; Luyue Chen; Jilong Liu; Yu Ren; Ming Yang; Anling ..., Cancer Research, 2013

The extensive involvement of miRNAs in cancer pathobiology has opened avenues for drug development based on oncomir inhibition. Dicer is the core enzyme in miRNA processing that cleaves the terminal loop of precursor microRNAs (pre-miRNAs) to generate mature miRNA duplexes. Using the ...


A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases [Biochemistry]

20-Aug-2013 | Quin H. Christensen; Tyler L. Grove; Squire J. Booker; E. Peter Greenberg, Proceedings of the National Academy of Sciences current issue, 2013

Many Proteobacteria use N-acyl-homoserine lactone (acyl-HSL) quorum sensing to control specific genes. Acyl-HSL synthesis requires unique enzymes that use S-adenosyl methionine as an acyl acceptor and amino acid donor. We developed and executed an enzyme-coupled high-throughput cell-free screen ...


A high throughput screen for antibiotic drug discovery

17-Aug-2013 | Thomas C. Scanlon, Sarah M. Dostal, Karl E. Griswold, Biotechnology and Bioengineering, 2013

Abstract We describe an ultra‐high throughput screening platform enabling discovery and/or engineering of natural product antibiotics. The methodology involves creation of hydrogel‐in‐oil emulsions in which recombinant microorganisms are co‐emulsified with bacterial pathogens; antibiotic ...


A high-content, high-throughput siRNA screen identifies cyclin D2 as a potent regulator of muscle progenitor cell fusion and a target to enhance muscle regeneration

15-Aug-2013 | Michael V. Khanjyan; Jonathan Yang; Refik Kayali; Thomas Caldwell; Carmen Bertoni, Human Molecular Genetics, 2013

Cell-mediated regenerative approaches using muscle progenitor cells hold promises for the treatment of many forms of muscle disorders. Their applicability in the clinic, however, is hindered by the low levels of regeneration obtained after transplantation and the large number of cells required to ...


Orthoformimycin, a Selective Inhibitor of Bacterial Translation Elongation from Streptomyces Containing an Unusual Orthoformate

15-Aug-2013 | Sonia I. Maffioli; Attilio Fabbretti; Letizia Brandi; Andreas Savelsbergh; Paolo Monciardini; Monica Abbondi; Rossan ..., ACS Chemical Biology, 2013

Upon high throughput screening of 6700 microbial fermentation extracts, we discovered a compound, designated orthoformimycin, capable of inhibiting protein synthesis in vitro with high efficiency. The molecule, whose structure was elucidated by chemical, spectrometric, and spectroscopic methods, ...


Switchable Reporter Enzymes Based on Mutually Exclusive Domain Interactions Allow Antibody Detection Directly in Solution

13-Aug-2013 | Sambashiva Banala; Stijn J.A. Aper; Werner Schalk; Maarten Merkx, ACS Chemical Biology, 2013

Detection of antibodies is essential for the diagnosis of many diseases including infections, allergies, and autoimmune diseases. Current heterogeneous immunoassays require multiple time-consuming binding and washing steps, which limits their application in point-of-care diagnostics and ...


Optimization of random PEGylation reactions by means of high throughput screening

12-Aug-2013 | Benjamin Maiser, Florian Dismer, Jürgen Hubbuch, Biotechnology and Bioengineering, 2013

ABSTRACT Since the first FDA approval of a PEGylated product in 1990, so called random PEGylation reactions are still used to increase the efficacy of biopharmaceuticals and represent the major technology of all approved PEG‐modified drugs. However, the great influence of process parameters ...


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