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129 Newest Publications about the topic gel filtration chromatography


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A Comparison of Methods To Enhance Protein Detection of Lipoproteins by Mass Spectrometry

12-Jun-2015 | Anna Heink, MS; W. Sean Davidson, PhD; Debi K Swertfeger; L. Jason Lu; Amy S Shah, MD MS, Journal of Proteome Research, 2015

We sought to develop a new method to more efficiently analyze lipid-bound proteins by mass spectrometry using a combination of a lipid removal agent (LRA) that selectively targets lipid-bound proteins and a mass spectrometry compatible detergent, anionic acid labile surfactant (AALS), that is ...


Intrinsic disorder of human Yin Yang 1 protein

12-May-2015 | Andrzej Górecki, Piotr Bonarek, Adam Kazimierz Górka, Małgorzata Figiel, Mateusz Wilamowski, Marta Dziedzicka‐Wasylewska, Proteins: Structure, Function, and Bioinformatics, 2015

Abstract YY1 (Yin Yang 1) is a zinc finger protein with an essential role in various biological functions via DNA– and protein–protein interactions with numerous partners. YY1 is involved in the regulation of a broad spectrum of cellular processes such as embryogenesis, proliferation, ...


Purification and characterization of a trypsin inhibitor from the seeds of Artocarpus heterophyllus Lam.

01-May-2015 | Junchen Lyu; Yuan Liu; Tianchen An; Yujun Liu; Manchuriga Wang; Yanting Song; Feifei Zheng; Dan Wu; Yingxia Zhang; S ..., Acta Biochimica et Biophysica Sinica, 2015

A proteinaceous inhibitor against trypsin was isolated from the seeds of Artocarpus heterophyllus Lam. by successive ammonium sulfate precipitation, ion-exchange, and gel-filtration chromatography. The trypsin inhibitor, named as AHLTI (A. heterophyllus Lam. trypsin inhibitor), consisted of a ...


A Comparison of the Mouse and Human Lipoproteome: Suitability of the Mouse Model for Studies of Human Lipoproteins

27-Apr-2015 | Scott M. Gordon; Hailong Li; Xiaoting Zhu; Amy S. Shah; L. Jason Lu; W. Sean Davidson, Journal of Proteome Research, 2015

Plasma levels of low density lipoproteins (LDL) and high density lipoproteins (HDL) exhibit opposing associations with cardiovascular disease in human populations and mouse models have been heavily used to derive a mechanistic understanding of these relationships. In humans, recent mass ...


Marine Drugs, Vol. 13, Pages 2580-2601: Influence of Amino Acid Compositions and Peptide Profiles on Antioxidant Capacities of Two Protein Hydrolysates from Skipjack Tuna (Katsuwonus pelamis) Dark Muscle

27-Apr-2015 | Chi, Chang-Feng ; Hu, Fa-Yuan ; Wang, Bin ; Li, Zhong-Rui ; Luo, Hong-Yu, Marine Drugs, 2015

Influence of amino acid compositions and peptide profiles on antioxidant capacities of two protein hydrolysates from skipjack tuna (Katsuwonus pelamis) dark muscle was investigated. Dark muscles from skipjack tuna were hydrolyzed using five separate proteases, including pepsin, trypsin, Neutrase, ...


Marine Drugs, Vol. 13, Pages 1993-2009: Two Novel Antioxidant Nonapeptides from Protein Hydrolysate of Skate (Raja porosa) Muscle

03-Apr-2015 | Hu, Fa-Yuan ; Chi, Chang-Feng ; Wang, Bin ; Deng, Shang-Gui, Marine Drugs, 2015

In the current study, the preparation conditions of neutrase hydrolysate (SMH) from skate (Raja porosa) muscle protein were optimized using orthogonal L9(3)4 tests, and R values indicated that pH was the most important factor affecting HO· scavenging activity of SMH. Under the optimum ...


Separation of angiotensin I‐converting enzyme inhibitory peptides from bovine connective tissue and their stability towards temperature, pH and digestive enzymes

02-Mar-2015 | Yu Fu, Jette F. Young, Trine K. Dalsgaard, Margrethe Therkildsen, International Journal of Food Science & Technology, 2015

Summary Bovine collagen was isolated from connective tissue, a by‐product in the meat processing industry and characterised by SDS‐PAGE. Alcalase and papain were employed to generate collagen hydrolysates with different degree of hydrolysis (DH). In vitro angiotensin I‐converting enzyme (ACE) ...


Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R‐phycoerythrin from red algae Bangia fusco‐purpurea

02-Mar-2015 | Qiang Wu, Xiao‐Ping Fu, Le‐Chang Sun, Qian Zhang, Guang‐Ming Liu, Min‐Jie Cao, Qiu‐Feng Cai, International Journal of Food Science & Technology, 2015

Summary R‐phycoerythrin (R‐PE) was purified from the red algae Bangia fusco‐purpurea after 35–50% ammonium sulphate fractionation followed by ion‐exchange column chromatography on DEAE‐Sepharose, resulting in a purity (A565/A280) ratio of 5.1. The circular dichroism spectroscopy results ...


Two Types of Functionally Distinct Fiber Containing Structural Protein Complexes Are Produced during Infection of Adenovirus Serotype 5

27-Feb-2015 | Bo Zhang et al., PLoS ONE, 2015

by Bo Zhang, Yuhua Yan, Jie Jin, Hongyu Lin, Zongyi Li, Xiaoyan Zhang, Jin Liu, Chao Xi, Andre Lieber, Xiaolong Fan, Liang Ran Adenoviruses are common pathogens. The localization of their receptors coxsackievirus and adenovirus receptor, and desmoglein-2 in cell-cell junction complexes between ...


A laboratory exercise for visible gel filtration chromatography using fluorescent proteins

17-Nov-2014 | Wenqiang Zhang, Yibin Cao, Lishan Xu, Jufang Gong, Meihao Sun, Biochemistry and Molecular Biology Education, 2014

Abstract Gel filtration chromatography (GFC) separates molecules according to size and is one of the most widely used methods for protein purification. Here, red fluorescent protein (RFP), green fluorescent protein (GFP), yellow fluorescent protein (YFP), cyan fluorescent protein (CFP), and/or ...


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