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129 Newest Publications about the topic gel filtration chromatography


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Sulfoureido Lipopeptides from the Marine Sponge Discodermia kiiensis

23-Aug-2016 | Karen Co Tan; Toshiyuki Wakimoto; Ikuro Abe, Journal of Natural Products, 2016

New N-sulfoureidylated lipopeptides, sulfolipodiscamides A–C (1–3), were isolated by gel filtration chromatography of the n-butanol fraction of the marine sponge Discodermia kiiensis. By extensive NMR analyses and high-resolution mass spectrometry, the structures of 1–3 were elucidated as having ...


Microwave‐Assisted Extraction, Chemical Structures, and Chain Conformation of Polysaccharides from a Novel Cordyceps Sinensis Fungus UM01

12-Aug-2016 | Kit‐Leong Cheong, Lan‐Ying Wang, Ding‐Tao Wu, De‐Jun Hu, Jing Zhao, Shao‐Ping Li, Journal of Food Science, 2016

Abstract Cordyceps sinensis is a well‐known tonic food with broad medicinal properties. The aim of the present study was to investigate the optimization of microwave‐assisted extraction (MAE) and characterize chemical structures and chain conformation of polysaccharides from a novel C. ...


Sphingosine 1‐phosphate and its carrier apolipoprotein M in human sepsis and in Escherichia coli sepsis in baboons

17-Mar-2016 | Cecilia Frej, Adam Linder, Kaisa E. Happonen, Fletcher B. Taylor, Florea Lupu, Björn Dahlbäck, Journal of Cellular and Molecular Medicine, 2016

Abstract Sphingosine 1‐phosphate (S1P) is an important regulator of vascular integrity and immune cell migration, carried in plasma by high‐density lipoprotein (HDL)‐associated apolipoprotein M (apoM) and by albumin. In sepsis, the protein and lipid composition of HDL changes dramatically. The ...


The Kub5-Hera/RPRD1B interactome: a novel role in preserving genetic stability by regulating DNA mismatch repair

29-Feb-2016 | Praveen L. Patidar; Edward A. Motea; Farjana J. Fattah; Yunyun Zhou; Julio C. Morales; Yang Xie; Harold R. Garner; D ..., Nucleic Acids Research, 2016

Ku70-binding protein 5 (Kub5)-Hera (K-H)/RPRD1B maintains genetic integrity by concomitantly minimizing persistent R-loops and promoting repair of DNA double strand breaks (DSBs). We used tandem affinity purification-mass spectrometry, co-immunoprecipitation and gel-filtration chromatography to ...


Antagonistic activity of endo‐β‐1,3‐glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids

17-Feb-2016 | Chatsuda Sakdapetsiri, Yasuhisa Fukuta, Yaovapa Aramsirirujiwet, Norifumi Shirasaka, Vichien Kitpreechavanich, Journal of Basic Microbiology, 2016

A total of 123 actinomycetes was isolated from 12 varieties of wild orchids and screened for potential antagonistic activity against Phytophthora, which causes black rot disease in orchids. In vitro and in vivo experimental results revealed that Streptomyces sp. strain 9X166 showed the highest ...


Urinary apolipoprotein M as a biomarker of acute kidney injury in children undergoing heart surgery

07-Dec-2015 | Eva Martha Madsen Svarrer; Henrik Ørbæk Andersen; Morten Helvind; Maartje CJ Slagman; Gerjan Navis; Robin PF Dullaar ..., Biomarkers in Medicine, 2015

Aim: To investigate whether apoM is excreted in urine of children undergoing heart surgery and the potential of apoM as early biomarker of acute kidney injury (AKI). Materials & methods: Urine was collected in children undergoing heart surgery. ApoM was measured with ELISA. U-apoM was ...


Characterization and In Vitro Antioxidant Activity of a Polysaccharide from Cordyceps sobolifera

20-Nov-2015 | Jike Lu, Guofeng Gu, Limin Hao, Zenan Jin, Xu Wang, Journal of Food Processing and Preservation, 2015

Abstract The characteristics and antioxidant activities of Cordyceps sobolifera polysaccharide (CSP) were investigated. Results from high‐performance gel filtration chromatography showed that the average molecular weight of CSP was 1.53 × 104 kDa, while this polysaccharide was mainly composed ...


Novel polyol‐responsive monoclonal antibodies against extracellular β ‐D‐glucans from Pleurotus ostreatus

19-Nov-2015 | Magda C. Semedo, Amin Karmali, Sónia Martins, Luís Fonseca, Biotechnology Progress, 2015

Abstract β‐D‐glucans from mushroom strains play a major role as biological response modifiers in several clinical disorders. Therefore, a specific assay method is of critical importance to find useful and novel sources of β‐D‐ glucans with anti‐tumor activity. Hybridoma technology was used to ...


Characterization and application of a novel bioemulsifier in crude oil degradation by Acinetobacter beijerinckii ZRS

18-Nov-2015 | Yi‐He Zhao, Li‐Yuan Chen, Zi‐Jing Tian, Yue Sun, Jin‐Biao Liu, Lei Huang, Journal of Basic Microbiology, 2015

Bioemulsifiers can be applicated in a variety of areas such as bioremediation and microbial‐enhanced oil recovery. The present study was aimed at bioemulsifier production, optimization, stability studies, and applications of the bioemulsifier produced by one of these strains, Acinetobacter ...


Viruses, Vol. 7, Pages 3891-3909: Optimization and Characterization of Candidate Strain for Coxsackievirus A16 Inactivated Vaccine

17-Jul-2015 | Li, Jingliang ; Liu, Guanchen ; Liu, Xin ; Yang, Jiaxin ; Chang, Junliang ; Zhang, Wenyan ; Yu, Xiao-Fang, Viruses, 2015

Coxsackievirus A16 (CA16) and enterovirus 71 (EV71), both of which can cause hand, foot and mouth disease (HFMD), are responsible for large epidemics in Asian and Pacific areas. Although inactivated EV71 vaccines have completed testing in phase III clinical trials in Mainland China, CA16 vaccines ...


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