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129 Newest Publications about the topic gel filtration chromatography

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Peanuts, Peanut Oil, and Fat Free Peanut Flour Reduced Cardiovascular Disease Risk Factors and the Development of Atherosclerosis in Syrian Golden Hamsters

01-May-2010 | Stephens, Amanda M.; Dean, Lisa L.; Davis, Jack P.; Osborne , Jason A.; Sanders, Timothy H., Journal of Food Science, 2010

Human clinical trials have demonstrated the cardiovascular protective properties of peanuts and peanut oil in decreasing total and low density lipoprotein cholesterol (LDL-C) without reducing high density lipoprotein cholesterol (HDL-C). The cardiovascular effects of the nonlipid portion of ...

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Isolation and preliminary characterization of a respiratory nitrate reductase from hydrocarbon-degrading bacterium Gordonia alkanivorans S7

09-Apr-2010 | Irena Romanowska, Ewa Kwapisz, Magdalena Mitka, Stanisław Bielecki, Journal of Industrial Microbiology and Biotechnology, 2010

Gordonia alkanivorans S7 is an efficient degrader of fuel oil hydrocarbons that can simultaneously utilize oxygen and nitrate as electron acceptors. The respiratory nitrate reductase (Nar) from this organism has been isolated using ion exchange chromatography and gel filtration, and then ...

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Inhibitory Activity by Barley Coffee Components Towards Streptococcus Mutans Biofilm

02-Apr-2010 | Monica Stauder, Adele Papetti, Maria Daglia, Luigi Vezzulli, Gabriella Gazzani, Pietro E. Varaldo, Carla Pruzzo, Current Microbiology, 2010

It was shown that barley coffee (BC) interferes with Streptococcus mutans adsorption to hydroxyapatite. After BC component fractionation by dialysis and gel filtration chromatography (GFC), it was found that the low molecular mass ( 1,000 kDa) melanoidin fraction display strong anti-adhesive ...

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Purification, characterization, structural analysis and protein chemistry of a buffalo heart galectin-1

31-Mar-2010 | Ghulam Md Ashraf, Sabika Rizvi, Shoa Naqvi, Nida Suhail, Nayeem Bilal, Shirin Hasan, Mohammad Tabish, Naheed Banu, Amino Acids, 2010

A soluble β-galactoside-binding lectin was purified by gel filtration chromatography from Bubalus bubalis heart. Its metal-independent nature, molecular weight of 14.5 kDa, preferential affinity for β-d-lactose, and 87–92% identity with carbohydrate recognition domain of previously reported ...

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Expression and Purification of Recombinant Human MCT-1 Oncogene in Insect Cells

15-Jan-2010 | Isabel Pérez-Arellano, Roberto Gozalbo-Rovira, Ana Isabel Martínez, Javier Cervera, The Protein Journal, 2010

MCT-1 protein is encoded by an oncogene highly expressed in lymphomas. It is implicated in the interaction with the cap complex of the mRNA, through an RNA binding domain, named PUA. Targeted suppression of this domain attenuates the malignant phenotype and hence MCT-1 is a potential target for ...

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Effects of bovine milk lactoperoxidase system on some bacteria

05-Jan-2010 | M. Çankaya, M. Şişecioğlu, Ö. Barış, M. Güllüce, H. Özdemir, Applied Biochemistry and Microbiology, 2010

Bovine lactoperoxidase (LPO) was purified from skimmed milk using amberlite CG-50-H+ resin, CM sephadex C-50 ion-exchange chromatography, and sephadex G-100 gel filtration chromatography. Lactoperoxidase was purified 20.45-fold with a yield of 28.8%. Purity of enzyme checked by sodium dodecyl ...

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Functional characterization of GDP-mannose pyrophosphorylase from Leptospira interrogans serovar Copenhageni

25-Dec-2009 | Matías D. Asención Diez, Ana Demonte, Jorge Giacomelli, Sergio Garay, Daniel Rodrígues, Birgit Hofmann, Hans-Juergue ..., Archives of Microbiology, 2009

Leptospira interrogans synthesizes a range of mannose-containing glycoconjugates relevant for its virulence. A prerequisite in the synthesis is the availability of the GDP-mannose, produced from mannose-1-phosphate and GTP in a reaction catalyzed by GDP-mannose pyrophosphorylase. The gene coding ...

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Purification and characterization of thermostable monomeric chloroplastic Cu/Zn superoxide dismutase from Chenopodium murale

28-Oct-2009 | Sabarinath Sundaram, Sunil Khanna, Renu Khanna-Chopra, Physiology and Molecular Biology of Plants, 2009

Superoxide dismutase is the first line of defense against oxidative stress and thus helps in maintaining the cellular integrity. Chenopodium murale, a weed species adapted to widely varying climatic conditions faces extremes of temperatures ranging from 4 °C to 45 °C (Tmax) during growth and ...

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THE INTERACTION OF PROTEIN AND POLYPHENOL SPECIES IN READY TO DRINK BLACK TEA LIQUOR PRODUCTION

20-May-2009 | D. WU, M. R. BIRD, Journal of Food Process Engineering, 2009

Haze and tea cream generation is a serious problem in the production of ready-to-drink (RTD) black tea liquors. Such precipitates consist primarily of proteins and polyphenols. Tea protein and tea polyphenol have been separated successfully in this study. The interactions of (1) tea protein and ...

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