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452 Newest Publications about the topic fluorescent proteins

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Titration of Lentivirus Vectors

01-Apr-2018 | Sena-Esteves, M., Gao, G., CSH Protocols, 2018

The titer of a lentivirus vector is often expressed in transducing units per milliliter. This is a functional titer that reflects the lentivirus’ ability to transduce a particular cell line under specific conditions. Transduction of other cell lines is likely to be different and will require ...

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Live Cell Visualization of Multiple Protein–Protein Interactions with BiFC Rainbow

11-Jan-2018 | Sheng Wang; Miao Ding; Boxin Xue; Yingping Hou; Yujie Sun, ACS Chemical Biology, 2018

As one of the most powerful tools to visualize PPIs in living cells, bimolecular fluorescence complementation (BiFC) has gained great advancement during recent years, including deep tissue imaging with far-red or near-infrared fluorescent proteins or super-resolution imaging with photochromic ...

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Systematic characterization of maturation time of fluorescent proteins in living cells

20-Nov-2017 | Enrique Balleza; J Mark Kim; Philippe Cluzel, Nature Methods, 2017

Systematic characterization of maturation time of fluorescent proteins in living cells Systematic characterization of maturation time of fluorescent proteins in living cells, Published online: 20 November 2017; doi:10.1038/nmeth.4509 This work characterizes the maturation kinetics of 50 cyan to ...

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Avoidance of reporter assay distortions from fused dual reporters [METHOD]

01-Aug-2017 | Gary Loughran; Michael T. Howard; Andrew E. Firth; John F. Atkins, RNA, 2017

Positioning test sequences between fused reporters permits monitoring of both translation levels and framing, before and after the test sequence. Many studies, including those on recoding such as productive ribosomal frameshifting and stop codon readthrough, use distinguishable luciferases or ...

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Efficient switching of mCherry fluorescence using chemical caging [Chemistry]

03-Jul-2017 | Bas M. C. Cloin; Elke De Zitter; Desiree Salas; Vincent Gielen; Gert E. Folkers; Marina Mikhaylova; Maike Bergeler; ..., Proceedings of the National Academy of Sciences current issue, 2017

Fluorophores with dynamic or controllable fluorescence emission have become essential tools for advanced imaging, such as superresolution imaging. These applications have driven the continuing development of photoactivatable or photoconvertible labels, including genetically encoded fluorescent ...

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Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores

06-Jun-2017 | Nina G. Bozhanova; Mikhail S. Baranov; Karen S. Sarkisyan; Roman Gritcenko; Konstantin S. Mineev; Svetlana V. Golodu ..., ACS Chemical Biology, 2017

Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric ...

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Blue fluorescent amino acid for biological spectroscopy and microscopy [Applied Biological Sciences]

06-Jun-2017 | Mary Rose Hilaire; Ismail A. Ahmed; Chun-Wei Lin; Hyunil Jo; William F. DeGrado; Feng Gai, Proceedings of the National Academy of Sciences current issue, 2017

Many fluorescent proteins are currently available for biological spectroscopy and imaging measurements, allowing a wide range of biochemical and biophysical processes and interactions to be studied at various length scales. However, in applications where a small fluorescence reporter is required ...

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The zebrafish embryo as a model to quantify early inflammatory cell responses to biomaterials

16-May-2017 | Xiaolin Zhang, Oliver. W. Stockhammer, Leonie de Boer, Norbert. O. E Vischer, Herman. P. Spaink, Dirk. W. Grijpma, S ..., Journal of Biomedical Materials Research Part A, 2017

Abstract In order to rapidly assess early inflammatory cell responses provoked by biomaterials in the full complexity of the living organism, we developed a zebrafish embryo model which allows real time analysis of these responses to biomaterial microspheres. Fluorescently labeled microspheres ...

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Novel Thermostable Flavin‐binding Fluorescent Proteins from Thermophilic Organisms

13-May-2017 | Marcus Wingen, Karl‐Erich Jaeger, Thomas Gensch, Thomas Drepper, Photochemistry and Photobiology, 2017

Abstract Flavin‐binding fluorescent proteins (FbFPs) are small, oxygen‐independent invivo reporters, derived from Light Oxygen Voltage (LOV) domains of photoreceptors. Here, we investigated the thermostability of existing, as well as novel FbFPs, whose genes were identified in genome ...

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Delineating morbillivirus entry, dissemination and airborne transmission by studying in vivo competition of multicolor canine distemper viruses in ferrets

08-May-2017 | Rory D. de Vries; Martin Ludlow; Alwin de Jong; Linda J. Rennick; R. Joyce Verburgh; Geert van Amerongen; Debby van ..., PLoS Pathogens, 2017

by Rory D. de Vries, Martin Ludlow, Alwin de Jong, Linda J. Rennick, R. Joyce Verburgh, Geert van Amerongen, Debby van Riel, Peter R. W. A. van Run, Sander Herfst, Thijs Kuiken, Ron A. M. Fouchier, Albert D. M. E. Osterhaus, Rik L. de Swart, W. Paul Duprex Identification of cellular receptors ...

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