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57 Newest Publications about the topic fluorescence lifetime

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Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging

22-Feb-2018 | Kaiyu Zheng; Thomas P Jensen; Dmitri A Rusakov, Nature Protocols, 2018

Nature Protocols 13, 581 (2018). doi:10.1038/nprot.2017.154 Authors: Kaiyu Zheng, Thomas P Jensen & Dmitri A Rusakov Nanomolar-range fluctuations of intracellular [Ca2+] are critical for brain cell function but remain difficult to measure. We have advanced a microscopy ...

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Fluorescence lifetime and UV‐Vis spectroscopy to evaluate the interactions between quercetin and its yeast microcapsule

09-Sep-2017 | Bao‐Ngoc Pham‐Hoang, Pascale Winckler, Yves Waché, Biotechnology Journal, 2017

Quercetin is a fragile bioactive compound. Several works have tried to preserve it by encapsulation but the form of encapsulation (mono‐ or supra‐molecular structure, tautomeric form), though important for stability and bioavailability, remains unknown. The present work aims at developing a ...

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Using Fluorescence Lifetime Microscopy to Study the Subcellular Localization of Anthocyanins

08-Aug-2016 | Alexandra Chanoca, Brian Burkel, Nik Kovinich, Erich Grotewold, Kevin W. Eliceiri, Marisa S. Otegui, The Plant Journal, 2016

Summary Anthocyanins are flavonoid pigments that accumulate in most seed plants. They are synthesized in the cytoplasm but accumulate inside the vacuoles. Anthocyanins are pigmented at the lower vacuolar pH, but in the cytoplasm they can be visualized based on their fluorescence properties. ...

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Fluorescence lifetime imaging of DAPI‐stained nuclei as a novel diagnostic tool for the detection and classification of B‐cell chronic lymphocytic leukemia

17-Jun-2016 | Gilad Yahav, Abraham Hirshberg, Ophira Salomon, Ninette Amariglio, Luba Trakhtenbrot, Dror Fixler, Cytometry Part A, 2016

Abstract B‐cell chronic lymphocytic leukaemia (B‐CLL) and B‐cell precursor acute lymphoblastic leukaemia (B‐ALL) are the most common type of leukaemia in adults and children, respectively. Today, fluorescence in situ hybridization (FISH) is the standard for detecting chromosomal aberrations ...

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Time lapse in vivo microscopy reveals distinct dynamics of microglia‐tumor environment interactions – a new role for the tumor perivascular space as highway for trafficking microglia

03-May-2016 | Simon Heinrich Bayerl, Raluca Niesner, Zoltan Cseresnyes, Helena Radbruch, Julian Pohlan, Susan Brandenburg, Marcus ..., Glia, 2016

Microglial cells are critical for glioma growth and progression. However, only little is known about intratumoral microglial behavior and the dynamic interaction with the tumor. Currently the scarce understanding of microglial appearance in malignant gliomas merely originates from histological ...

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Fluorescent Bioactive Corrole Grafted-Chitosan Films

11-Mar-2016 | Joana F. B. Barata; Ricardo J. B. Pinto; Vanda I. R. C. Vaz Serra; Armando J. D. Silvestre; Tito Trindade; Maria Gra ..., Biomacromolecules, 2016

Transparent corrole grafted-chitosan films were prepared by chemical modification of chitosan with a corrole macrocycle, namely, 5,10,15-tris(pentafluorophenyl)corrole (TPFC), followed by solvent casting. The obtained films were characterized in terms of absorption spectra (UV–vis), FLIM ...

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A genetic and physical study of the interdomain linker of E. coli AraC protein‐a trans‐subunit communication pathway

22-Jan-2016 | Fabiana Malaga, Ory Mayberry, David J. Park, Michael E. Rodgers, Dmitri Toptygin, Robert F. Schleif, Proteins: Structure, Function, and Bioinformatics, 2016

Abstract Genetic experiments with full length AraC and biophysical experiments with its dimerization domain plus linker suggest that arabinose binding to the dimerization domain changes the properties of the inter‐domain linker which connects the dimerization domain to the DNA binding domain ...

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Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging

11-Dec-2015 | Travis Omer et al., PLoS ONE, 2015

by Travis Omer, Xavier Intes, Juergen Hahn Fluorescence lifetime imaging (FLIM) when paired with Förster resonance energy transfer (FLIM-FRET) enables the monitoring of nanoscale interactions in living biological samples. FLIM-FRET model-based estimation methods allow the quantitative retrieval ...

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Direct evidence of mitochondrial G-quadruplex DNA by using fluorescent anti-cancer agents

02-Dec-2015 | Wei-Chun Huang; Ting-Yuan Tseng; Ying-Ting Chen; Cheng-Chung Chang; Zi-Fu Wang; Chiung-Lin Wang; Tsu-Ning Hsu; Pei-T ..., Nucleic Acids Research, 2015

G-quadruplex (G4) is a promising target for anti-cancer treatment. In this paper, we provide the first evidence supporting the presence of G4 in the mitochondrial DNA (mtDNA) of live cells. The molecular engineering of a fluorescent G4 ligand, 3,6-bis(1-methyl-4-vinylpyridinium) carbazole ...

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Subcellular Partitioning of Protein Tyrosine Phosphatase 1B to the Endoplasmic Reticulum and Mitochondria Depends Sensitively on the Composition of Its Tail Anchor

02-Oct-2015 | Julia Fueller et al., PLoS ONE, 2015

by Julia Fueller, Mikhail V. Egorov, Kirstin A. Walther, Ola Sabet, Jana Mallah, Markus Grabenbauer, Ali Kinkhabwala The canonical protein tyrosine phosphatase PTP1B is an important regulator of diverse cellular signaling networks. PTP1B has long been thought to exert its influence solely from ...

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