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663 Newest Publications about the topic electrophoresis

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Accurate detection of chemical modifications in RNA by mutational profiling (MaP) with ShapeMapper 2 [BIOINFORMATICS]

01-Feb-2018 | Steven Busan; Kevin M. Weeks, RNA, 2018

Mutational profiling (MaP) enables detection of sites of chemical modification in RNA as sequence changes during reverse transcription (RT), subsequently read out by massively parallel sequencing. We introduce ShapeMapper 2, which integrates careful handling of all classes of adduct-induced ...

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Comparative Proteomics Studies of Insect Cuticle by Tandem Mass Spectrometry: Application of a Novel Proteomics Approach to the Pea Aphid Cuticular Proteins

12-Jan-2018 | Masson Victor, Arafah Karim, Voisin Sébastien, Bulet Philippe, PROTEOMICS, 2018

Abstract The exoskeleton of arthropods, called cuticle, is a biological composite material consisting principally of N–acetylglucosamine polymer embedded in cuticular proteins (CPs). The CPs have been studied and characterized by mass spectrometry in several cuticular structures and in many ...

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Integrated analysis of the transcriptome and metabolome of Corynebacterium glutamicum during penicillin‐induced glutamic acid production

11-Jan-2018 | Takashi Hirasawa, Masaki Saito, Katsunori Yoshikawa, Chikara Furusawa, Hiroshi Shmizu, Biotechnology Journal, 2018

Corynebacterium glutamicum is known for its ability to produce glutamic acid and has been utilized for the fermentative production of various amino acids. Glutamic acid production in C. glutamicum is induced by penicillin. In this study, we analyzed the transcriptome and metabolome of C. ...

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Integrated analysis of the transcriptome and metabolome of Corynebacterium glutamicum during penicillin‐induced glutamic acid production

11-Jan-2018 | Takashi Hirasawa, Masaki Saito, Katsunori Yoshikawa, Chikara Furusawa, Hiroshi Shmizu, Biotechnology Journal, 2018

Corynebacterium glutamicum is known for its ability to produce glutamic acid and has been utilized for the fermentative production of various amino acids. Glutamic acid production in C. glutamicum is induced by penicillin. In this study, we analyzed the transcriptome and metabolome of C. ...

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Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples

03-Nov-2017 | Martín E. Mautner; Agustín Pérez Santángelo; Rodrigo M. Corti Bielsa; Andrea Sala; Santiago Ginart; Daniel Corach, PLoS ONE, 2017

by Martín E. Mautner, Agustín Pérez Santángelo, Rodrigo M. Corti Bielsa, Andrea Sala, Santiago Ginart, Daniel Corach Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances ...

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Analysis of O-Glycopeptides by Acetone Enrichment and Capillary Electrophoresis-Mass Spectrometry

10-Oct-2017 | Montserrat Mancera-Arteu; Estela Giménez; Fernando Benavente; José Barbosa; Victòria Sanz-Nebot, Journal of Proteome Research, 2017

Acetone precipitation was evaluated as a rapid, simple, low-cost, and efficient method for the selective purification of O-glycopeptides from enzymatic digests of glycoproteins. Ovalbumin (OVA), human and bovine α1-acid glycoprotein (hAGP and bAGP), human apolipoprotein C–III (APO-C3), and ...

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Synthesis, Quality Control and Bio‐evaluation of 99mTc‐cyclophosphamide

08-Sep-2017 | Tanveer Hussain Bokhari, Samina Roohi, Saira Hina, Muhammad Usman Akbar, Muhammad Sohaib, Munawar Iqbal, Chemical Biology & Drug Design, 2017

Abstract Cancer is found to be the leading cause of death worldwide characterized by uncontrolled cell division. Nuclear medicines imaging by using radiopharmaceuticals have pronounced potential for the diagnosis and treatment of cancers. Cyclophosphamide (CPH) is an antineoplastic drug which ...

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Aldo‐keto reductase and alcohol dehydrogenase contribute to benznidazole natural resistance in Trypanosoma cruzi

08-Sep-2017 | Laura González, Paola García‐Huertas, Omar Triana‐Chávez, Gabriela Andrea García, Silvane Maria Fonseca Murta, Ana M ..., Molecular Microbiology, 2017

Summary The improvement of Chagas disease treatment is focused not only on the development of new drugs but also in understanding mechanisms of action and resistance to drugs conventionally used. Thus, some strategies aim to detect specific changes in proteins between sensitive and resistant ...

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Chromatographic approaches for the characterization and quality control of therapeutic oligonucleotide impurities

04-Sep-2017 | N.M. El Zahar, N. Magdy, A.M. El‐Kosasy, Michael G. Bartlett, Biomedical Chromatography, 2017

Abstract Phosphorothioate (PS) oligonucleotides are a rapidly rising class of drugs with significant therapeutic applications. However, due to their complex structure and multi‐step synthesis and purification processes, generation of low level impurities and degradation products are common. ...

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And yet it is modified – Holding a candle to the dark matter of white matter

29-Aug-2017 | George Harauz, PROTEOMICS, 2017

The multilamellar membrane myelin sheath of the central nervous system (CNS), that enwraps axons to facilitate saltatory conduction in higher vertebrates, is held together by myelin basic protein (MBP). Yet this generalization masks how enigmatic MBP is, much like cosmological “dark matter”. ...

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