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34 Newest Publications about the topic assay development


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Standardisation of inactivated influenza vaccines – learning from history

21-Jan-2018 | John M Wood, Jerry P Weir, Influenza and Other Respiratory Viruses, 2018

Abstract The single radial immunodiffusion assay has been the accepted method for determining the potency of inactivated influenza vaccines since 1978. The world‐wide adoption of this assay for vaccine standardisation was facilitated through collaborative studies that demonstrated a high level ...


Quantitative proteomics of breast tumors: Tissue quality assessment to clinical biomarkers

27-Jan-2017 | Yi Chen, David Britton, Elizabeth R. Wood, Stephen Brantley, Anthony Magliocco, Ian Pike, John M. Koomen, PROTEOMICS, 2017

Liquid chromatography‐selected reaction monitoring mass spectrometry (LC‐SRM) is not only a proven tool for clinical chemistry, but also a versatile method to enhance the capability to quantify biomarkers for tumor biology research. As the treatment of cancer continues to evolve, the ability to ...


Bioanalytical assay development and validation for simultaneous quantification of five schisandra lignans in rat primary hepatocytes based on LC‐MS/MS: application to a real‐time uptake study for Schisandra Lignan Extract

27-Jul-2016 | Dian Kang, Yuhao Shao, Xiaoxi Yin, Jingcheng Xiao, Tai Rao, Boyu Shen, Huimin Chen, Zhangpei Zhu, Guangji Wang, Yan ..., Biomedical Chromatography, 2016

Abstract Schisandra lignans, mainly including schizandrol A, schizandrol B, schisantherin A, schizandrin A, schizandrin B, etc, are the major active ingredients of Schisandra chinensis. In the present study, a robust liquid chromatography–tandem mass spectrometric (LC–MS/MS) method was ...


Data-Driven Approach To Determine Popular Proteins for Targeted Proteomics Translation of Six Organ Systems

19-Jul-2016 | Maggie P. Y. Lam; Vidya Venkatraman; Yi Xing; Edward Lau; Quan Cao; Dominic C. M. Ng; Andrew I. Su; Junbo Ge; Jennif ..., Journal of Proteome Research, 2016

Amidst the proteomes of human tissues lie subsets of proteins that are closely involved in conserved pathophysiological processes. Much of biomedical research concerns interrogating disease signature proteins and defining their roles in disease mechanisms. With advances in proteomics ...


Multiplexed data independent acquisition (MSX‐DIA) applied by high resolution mass spectrometry improves quantification quality for the analysis of histone peptides

19-May-2016 | Simone Sidoli, Rina Fujiwara, Benjamin A. Garcia, PROTEOMICS, 2016

We present the mass spectrometry (MS) based application of the innovative, although scarcely exploited, multiplexed data‐independent acquisition (MSX‐DIA) for the analysis of histone post‐translational modifications (PTMs). Histones are golden standard for complexity in MS based proteomics, due ...


Cell based assays for anti-plasmodium activity evaluation

10-Jan-2016 | Author(s): Thabang Mokgethi-Morule, David D. N'Da, European Journal of Pharmaceutical Science, 2016

Publication date: Available online 8 January 2016 Source:European Journal of Pharmaceutical Sciences Author(s): Thabang Mokgethi-Morule, David D. N'Da Malaria remains one of the most common and deadly infectious diseases worldwide. The severity of this global public health challenge is ...


Development of a Selected Reaction Monitoring (SRM) method for absolute quantitation of MYDGF/C19orf10 protein

05-Nov-2015 | Ravi C Dwivedi, Oleg V Krokhin, Hani S El‐Gabalawy, John A Wilkins, PROTEOMICS, 2015

Purpose To develop a mass spectrometry based scheduled reaction monitoring (SRM) assay for quantitation of Myeloid derived growth factor (MYGDF) formerly Chromosome 19 open reading frame (C19orf10) Experimental design Candidate reporter peptides were identified in digests of recombinant ...


Evaluation of assay interference and interpretation of CXCR4 receptor occupancy results in a preclinical study with MEDI3185, a fully human antibody to CXCR4

19-Sep-2015 | Martin Schwickart, Carlos Chavez, Simon Henderson, Inna Vainshtein, Nathan Standifer, Christopher DelNagro, Freshta ..., Cytometry Part B: Clinical Cytometry, 2015

Abstract Background Receptor occupancy (RO) assays provide a means to measure the direct interaction of therapeutics with their cell surface targets. Free receptor assays quantify cell‐surface receptors not bound by a therapeutic while total receptor assays quantify the amount of target on ...


Site selective syntheses of [3H]omeprazole using hydrogen isotope exchange chemistry

18-Sep-2015 | Scott R. Pollack, David J. Schenk, Journal of Labelled Compounds and Radiopharmaceuticals, 2015

Omeprazole (Prilosec®) is a selective and irreversible proton pump inhibitor used to treat various medical conditions related to the production of excess stomach acids. It functions by suppressing secretion of those acids. Radiolabeled compounds are commonly employed in the drug discovery and ...


Mushroom tyrosinase: A model system to combine experimental investigation of enzyme‐catalyzed reactions, data handling using R, and enzyme‐inhibitor structural studies

01-Sep-2015 | Robert Nairn, Will Cresswell, Jacqueline Nairn, Biochemistry and Molecular Biology Education, 2015

Abstract The activity of mushroom tyrosinase can be measured by monitoring the conversion of phenolic compounds into quinone derivatives using spectrophotometry. This article describes a series of experiments which characterize the functional properties of tyrosinase, the analysis of the ...


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