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3,415 Newest Publications in journal of proteome research

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Proteome of Bovine Mitochondria and Rod Outer Segment Disks: Commonalities and Differences

18-Jan-2018 | Maurizio Bruschi; Andrea Petretto; Federico Caicci; Martina Bartolucci; Daniela Calzia; Laura Santucci; Lucia Manni; ..., Journal of Proteome Research, 2018

The retinal rod outer segment (OS) is a stack of disks surrounded by the plasma membrane, housing proteins related to phototransduction, as well as mitochondrial proteins involved in oxidative phosphorylation (OxPhos). This prompted us to compare the proteome of bovine OS disks and mitochondria ...

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Novel Kunitz-like Peptides Discovered in the Zoanthid Palythoa caribaeorum through Transcriptome Sequencing

12-Jan-2018 | Qiwen Liao; Shengnan Li; Shirley Weng In Siu; Binrui Yang; Chen Huang; Judy Yuet-Wa Chan; Jean-Étienne R. L. Morligh ..., Journal of Proteome Research, 2018

Palythoa caribaeorum (class Anthozoa) is a zoanthid that together jellyfishes, hydra, and sea anemones, which are venomous and predatory, belongs to the Phyllum Cnidaria. The distinguished feature in these marine animals is the cnidocytes in the body tissues, responsible for toxin production and ...

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Reverse and Random Decoy Methods for False Discovery Rate Estimation in High Mass Accuracy Peptide Spectral Library Searches

11-Jan-2018 | Zheng Zhang; Meghan Burke; Yuri A. Mirokhin; Dmitrii V. Tchekhovskoi; Sanford P. Markey; Wen Yu; Raghothama Chaerkad ..., Journal of Proteome Research, 2018

Spectral library searching (SLS) is an attractive alternative to sequence database searching (SDS) for peptide identification due to its speed, sensitivity, and ability to include any selected mass spectra. While decoy methods for SLS have been developed for low mass accuracy peptide spectral ...

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VDAC1 and SERCA3 Mediate Progesterone-Triggered Ca2+ Signaling in Breast Cancer Cells

22-Dec-2017 | Juberiya M. Azeez; Ravindran Vini; Viji Remadevi; Arun Surendran; Abdul Jaleel; T. R. Santhosh Kumar; S. Sreeja, Journal of Proteome Research, 2017

Progesterone is a biphasic hormone whose confounding role in breast cancer cells involves an initial proliferative surge, followed by sustained growth arrest. Recently we reported that progesterone induces a time- and concentration-dependent release of reactive oxygen species and thus regulates ...

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Comparison of Internal Standard Approaches for SRM Analysis of Alpha-Synuclein in Cerebrospinal Fluid

21-Dec-2017 | Patrick Oeckl; Petra Steinacker; Markus Otto, Journal of Proteome Research, 2017

Absolute protein quantification by selected reaction monitoring (SRM, also MRM) is an alternative to immunoassays, and the gold standard here is the addition of stable-isotope labeled (SIL) proteins (PSAQ). Cerebrospinal fluid (CSF) is the preferred source of biomarkers for neurological diseases, ...

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Performance Evaluation of the Q Exactive HF-X for Shotgun Proteomics

20-Dec-2017 | Christian D. Kelstrup; Dorte B. Bekker-Jensen; Tabiwang N. Arrey; Alexander Hogrebe; Alexander Harder; Jesper V. Olsen, Journal of Proteome Research, 2017

Progress in proteomics is mainly driven by advances in mass spectrometric (MS) technologies. Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics applications. A new peak-picking algorithm, a ...

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Omics-Based Platform for Studying Chemical Toxicity Using Stem Cells

20-Dec-2017 | Yan Han; Jinghua Zhao; Ruili Huang; Menghang Xia; Daojing Wang, Journal of Proteome Research, 2017

The new strategy for chemical toxicity testing and modeling is to use in vitro human cell-based assays in conjunction with quantitative high-throughput screening (qHTS) technology, to identify molecular mechanisms and predict in vivo responses. Stem cells are more physiologically relevant than ...

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Characterization and Detection of Erythropoietin Fc Fusion Proteins Using Liquid Chromatography–Mass Spectrometry

19-Dec-2017 | Natalia V. Mesonzhnik; Pavel V. Postnikov; Svetlana A. Appolonova; Grigory I. Krotov, Journal of Proteome Research, 2017

Erythropoietin Fc (EPO-Fc) fusion proteins are potential drug candidates that have been designed for the treatment of anemia in humans by stimulating erythrocyte production. Such compounds can be considered performance-enhancing agents that may be used by athletes in endurance sports. This study ...

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Poisson Model To Generate Isotope Distribution for Biomolecules

19-Dec-2017 | Rovshan G. Sadygov, Journal of Proteome Research, 2017

We introduce a simplified computational algorithm for computing isotope distributions (relative abundances and masses) of biomolecules. The algorithm is based on Poisson approximation to binomial and multinomial distributions. It leads to a small number of arithmetic operations to compute isotope ...

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Multiple Reaction Monitoring for the Quantitation of Serum Protein Glycosylation Profiles: Application to Ovarian Cancer

19-Dec-2017 | Suzanne Miyamoto; Carol D. Stroble; Sandra Taylor; Qiuting Hong; Carlito B. Lebrilla; Gary S. Leiserowitz; Kyoungmi ..., Journal of Proteome Research, 2017

Protein glycosylation fingerprints are widely recognized as potential markers for disease states, and indeed differential glycosylation has been identified in multiple types of autoimmune diseases and several types of cancer. However, releasing the glycans leave the glycoproteins unknown; ...

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