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1,103 Newest Publications in biotechnology journal

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A fluorescence‐based sensor assay that monitors general protein aggregation in human cells

18-Jan-2018 | Marisa Pereira, Diogo Tomé, Ana S. Domingues, Ana S. Varanda, Cristiana Paulo, Manuel A. S. Santos, Ana R. Soares, Biotechnology Journal, 2018

Protein conformational disorders are characterized by disruption of protein folding and toxic accumulation of protein aggregates. Here we describe a sensitive and simple method to follow and monitor general protein aggregation in human cells. Heat shock protein 27 (HSP27) is an oligomeric small ...

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A fluorescence‐based sensor assay that monitors general protein aggregation in human cells

18-Jan-2018 | Marisa Pereira, Diogo Tomé, Ana S. Domingues, Ana S. Varanda, Cristiana Paulo, Manuel A. S. Santos, Ana R. Soares, Biotechnology Journal, 2018

Protein conformational disorders are characterized by disruption of protein folding and toxic accumulation of protein aggregates. Here we describe a sensitive and simple method to follow and monitor general protein aggregation in human cells. Heat shock protein 27 (HSP27) is an oligomeric small ...

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Integration of Gas Enhanced Oil Recovery in multiphase fermentations for the microbial production of fuels and chemicals

18-Jan-2018 | Susana Pedraza‐de Cuesta, Lore Keijzers, Luuk A.M. Wielen, Maria C. Cuellar, Biotechnology Journal, 2018

In multiphase fermentations where the product forms a second liquid phase or where solvents are added for product extraction, turbulent conditions disperse the oil phase as droplets. Surface‐active components (SACs) present in the fermentation broth can stabilize the product droplets thus ...

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Modeling the downstream processing of monoclonal antibodies reveals cost advantages for continuous methods for a broad range of manufacturing scales

17-Jan-2018 | Jonathan Hummel, Mark Pagkaliwangan, Xhorxhi Gjoka, Terence Davidovits, Rick Stock, Thomas Ransohoff, Rene Gantier, ..., Biotechnology Journal, 2018

The biopharmaceutical industry is evolving in response to changing market conditions, including increasing competition and growing pressures to reduce costs. Single‐use technologies and continuous bioprocessing have attracted attention as potential facilitators of cost‐optimized manufacturing ...

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LC/MS‐based Intact IgG and released glycan analysis for bioprocessing applications

17-Jan-2018 | Tianhua Wang, Kong Meng Hoi, Henning Stöckmann, Corrine Wan, Lyn Chiin Sim, Noor Hayati Bte Kamari, Shi Jie Tay, Ce ..., Biotechnology Journal, 2018

Robust plate based antibody glycan analysis platforms are urgently needed for biopharmaceutical development and manufacturing as well as for clinical biomarker research. A 96‐well plate based workflow has been developed to analyze both intact IgG antibodies and released N‐glycans using an ...

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Co‐expression of ORFCma with PHB depolymerase (PhaZCma) in Escherichia coli induces efficient whole‐cell biodegradation of polyesters

16-Jan-2018 | Ming‐Chieh Lee, En‐Jung Liu, Cheng‐Han Yang, Li‐Jung Hsiao, Tzong‐Ming Wu, Si‐Yu Li, Biotechnology Journal, 2018

Whole‐cell degradation of polyesters not only avoids the tedious process of enzyme separation, but also allows the degraded product to be reused as a carbon source. In this study, Escherichia coli BL21(DE3) harboring phaZCma, a gene encoding poly(3‐hydroxybutyrate) (PHB) depolymerase from ...

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Donor variability in growth kinetics of healthy hMSCs using manual processing: considerations for manufacture of cell therapies

15-Jan-2018 | Giulia Detela, Owen W Bain, Hae‐Won Kim, David J Williams, Chris Mason, Anthony Mathur, Ivan B Wall, Biotechnology Journal, 2018

Human mesenchymal stromal cells (hMSCs) are excellent candidates for cell therapy but their expansion to desired clinical quantities can be compromised by ex vivo processing, due to differences between donor material and process variation. The aim of this article was to characterize growth ...

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Defined serum free medium for bioreactor culture of an immortalized human erythroblast cell line

13-Jan-2018 | Esmond Lee, Zhong Ri Lim, Hong Yu‐Chen, Bin Xia Yang, Alan Tin‐Lun Lam, Allen Kuan‐Liang Chen, Jaichandran Sivalinga ..., Biotechnology Journal, 2018

Anticipated shortages in donated blood supply have prompted investigation of alternative approaches for in‐vitro production of red blood cells (RBCs), such as expansion of conditional immortalization erythroid progenitors. However, there is a bioprocessing challenge wherein factors promoting ...

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Changes in chromosome counts and patterns in CHO cell lines upon generation of recombinant cell lines and subcloning

12-Jan-2018 | Sabine Vcelar, Michael Melcher, Norbert Auer, Astrid Hrdina, Anja Puklowski, Friedrich Leisch, Vaibhav Jadhav, Till ..., Biotechnology Journal, 2018

Chinese hamster ovary cells are the number one production system for therapeutic proteins. A pre‐requirement for their use in industrial production of biopharmaceuticals is to be clonal, thus originating from a single cell in order to be phenotypically and genomically identical. In the present ...

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Computer simulation elucidates yeast flocculation and sedimentation for efficient industrial fermentation

12-Jan-2018 | Chen‐Guang Liu, Zhi‐Yang Li, Yue Hao, Juan Xia, Feng‐Wu Bai, Muhammad Aamer Mehmood, Biotechnology Journal, 2018

Flocculation plays an important role in the immobilized fermentation of biofuels and biochemicals. It is essential to understand the flocculation phenomenon at physical and molecular scale; however, flocs can't be studied directly due to fragile nature. Hence, the present study was focused on ...

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