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A New Validated HPLC Method for the Determination of Quercetin: Application to Study Pharmacokinetics in Rats


A simple, accurate, and reproducible High Performance Liquid Chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20A HPLC system equipped with a Supelcosil LC‐18 T C18 column and an isocratic mobile phase consisted of 0.3% trichloroacetic acid in water and acetonitrile HPLC grade (50:50, v/v) run at a flow rate of 0.9 ml/min for 13 minutes. The UV detection wavelength was set at 254 nm. The method exhibited good linearity (R2 > 0.994) over the assayed concentration range (0.10‐25 µg/ml) and demonstrated good intra‐day and inter‐day precision and accuracy (relative standard deviations and the deviation from predicted values were less than 20%). This method was also successfully applied for studying the pharmacokinetics of QR in rats following a single oral dose of QR to evaluate its pharmacokinetic parameters in rats.

Authors:   Khaled S. Abdelkawy, Mark E. Balyshev, Fawzy Elbarbry
Journal:   Biomedical Chromatography
Year:   2016
Pages:   n/a
DOI:   10.1002/bmc.3819
Publication date:   24-Aug-2016
Facts, background information, dossiers
  • UV detection
  • Single
  • quercetin
  • Acetonitrile
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