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Bioanalytical assay development and validation for simultaneous quantification of five schisandra lignans in rat primary hepatocytes based on LC‐MS/MS: application to a real‐time uptake study for Schisandra Lignan Extract

Abstract

Schisandra lignans, mainly including schizandrol A, schizandrol B, schisantherin A, schizandrin A, schizandrin B, etc, are the major active ingredients of Schisandra chinensis. In the present study, a robust liquid chromatography–tandem mass spectrometric (LC–MS/MS) method was developed for the simultaneous quantification of schisandra lignans in rat primary hepatocytes. Lovastatin was used as an internal standard, and chromatographic separation was achieved on a Shimadzu C18 column with a gradient elution at the flow rate of 0.2 ml/min. All the analytes were detected in multiple reaction monitoring mode (MRM) with positive electrospray ionization (ESI) since sodium adduct ion [M + Na]+ was observed as the most intensive peak in the MS spectrum. For schizandrol A, schisantherin A and schizandrin A, the dynamic range was within 2 to 1000 ng/mg · protein, and the linear range of schizandrol B and schizandrin B was from 5 to 1000 ng/mg · protein. The intra‐ and inter‐day precision was less than 15 % and the accuracy (RE %) ranged from ‐15 % to 15 %. No significant variation was observed in the stability tests. The validated method was then successfully applied to the time‐dependent uptake study for the Schisandra Lignan Extract (SLE) in rat primary hepatocytes.

Authors:   Dian Kang, Yuhao Shao, Xiaoxi Yin, Jingcheng Xiao, Tai Rao, Boyu Shen, Huimin Chen, Zhangpei Zhu, Guangji Wang, Yan Liang
Journal:   Biomedical Chromatography
Year:   2016
Pages:   n/a
DOI:   10.1002/bmc.3797
Publication date:   27-Jul-2016
Facts, background information, dossiers
  • reaction monitoring
  • lovastatin
  • flow
  • active ingredients
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