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Distribution of glucosinolates in camelina seed fractions by HPLC‐ESI‐MS/MS

Abstract

High glucosinolate concentrations were detected and identified in camelina (Camelina sativa L. Crantz.) seed fractions using reversed phase high performance liquid chromatography‐electrospray ionization‐mass spectrometry (HPLC‐ESI‐MS) in multiple reaction monitoring mode. Total glucosinolate quantitation was performed using proton nuclear magnetic resonance spectrometry using N,N‐dimethylformamide as an internal standard. Individual glucosinolate concentrations were determined by analysis on extracted ion chromatograms generated by a MS detector. Distribution of glucosinolates in fractions of C. sativa seeds during pressing is reported.

Practical Applications: HPLC‐ESI‐MS and HPLC‐ESI‐MS/MS using monolithic HPLC columns were used to detect, quantify, and identify the three major glucosinolates in Camelina sativa L. and fractions of C. sativa produced by processing. Quantitative extracted ion MS chromatograms afforded excellent quantitation of individual glucosinolates. The use of monolithic HPLC columns enabled rapid analysis of these samples.

Authors:   Deyun Yuan, Youn Young Shim, Jianheng Shen, Pramodkumar D. Jadhav, Venkatesh Meda, Martin J. T. Reaney
Journal:   European Journal of Lipid Science and Technology
Year:   2016
Pages:   n/a
DOI:   10.1002/ejlt.201600040
Publication date:   03-May-2016
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