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A scale‐down mimic for mapping the process performance of centrifugation, depth and sterile filtration


In the production of biopharmaceuticals disk‐stack centrifugation is widely used as a harvest step for the removal of cells and cellular debris. Depth filters followed by sterile filters are often then deployed to remove residual solids remaining in the centrate. Process development of centrifugation is usually conducted at pilot‐scale so as to mimic the commercial scale equipment but this method requires large quantities of cell culture and significant levels of effort for successful characterisation. A scale‐down approach based upon the use of a shear device and a bench‐top centrifuge has been extended in this work towards a preparative methodology that successfully predicts the performance of the continuous centrifuge and polishing filters. The use of this methodology allows the effects of cell culture conditions and large‐scale centrifugal process parameters on subsequent filtration performance to be assessed at an early stage of process development where material availability is limited. This article is protected by copyright. All rights reserved

Authors:   Adrian Joseph, Brian Kenty, Michael Mollet, Kenneth Hwang, Steven Rose, Stephen Goldrick, Jean Bender, Suzanne S. Farid, Nigel Titchener‐Hooker
Journal:   Biotechnology and Bioengineering
Year:   2016
Pages:   n/a
DOI:   10.1002/bit.25967
Publication date:   01-Mar-2016
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