Micropropagation of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers
A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1 mg l−1) or kinetin (0.5 mg l−1). BA was recorded to be better than kinetin in terms of number of days taken to bud break. The maximum number of shoots per explant was observed on 0.1 mg l−1 BA and 0.5 mg l−1 kinetin. Shoot proliferation decreased with increasing concentration of BA alone, but in case of a combination of BA and NAA (0.1 mg l−1 each), it increased with increasing concentration of BA up to 10.0 mg l−1. None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation. In the case of a combination of BA + kinetin + IBA, the maximum (5.5 shoots per explants) proliferation was observed on MS medium containing 1.0 mg l−1 BA + 0.5 mg l−1 kinetin + 0.5 mg l−1 IBA or 0.25 mg l−1 BA + 1.0 mg l−1 kinetin + 1.0 mg l−1 IBA. Regenerated shoots showed root induction on MS basal medium or on MS medium containing 1.0 mg l−1 IBA. It is concluded that a five-fold increase (1.0 mg l−1 BA + 0.5 mg l−1 kinetin + 0.5 mg l−1 IBA) in axillary shoot proliferation, while seven-fold increase (0.25 mg/l mg l−1 BA + 1.0 mg l−1 kinetin + 1.0 mg l−1 IBA) during the second cycle of multiplication could be obtained using the two plant growth regulator combinations. PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication.
Komal Goswami, R. Sharma, P. K. Singh, Govind Singh
Endothelial nitric oxide synthase polymorphism G298T in association with oxidative DNA damage in coronary atherosclerosis
Content Type Journal Article
Category Research Note
RAJESH G. KUMAR, Department of Genetics, Osmania Universi ... more
The objective of this study was to evaluate the activity, kinetic behavior, and thermal inactivation kinetics of peroxidase (POD) in aqueous extracts from two kinds of milk ripe stage corn, sweet corn and waxy corn. Optimum activities using guaiacol as the hydrogen donor were obtained for s ... more
Neutron imaging is a promising technique to study drying processes in food engineering as it is a non-intrusive, non-destructive technique, which provides quasi-real-time quantitative information of the water loss during drying and of the internal water distribution, at a high spatial and d ... more
Researchers at Washington State University report a new approach for the effective capture of tumor-derived exosomes from a prostate cancer cell line. Exosomes are small secreted vesicles that play a key role in intercellular communication and cancer progression.
Developing effective and hi ... more
In shampoo ads, hair always looks like a shiny, smooth surface. But for physicists peering into microscopes, the hair surface looks much more rugged, as it is made of saw-tooth, ratchet-like scales. In a new theoretical study published, Matthias Radtke and Roland Netz have demonstrated that ... more