Complement profile and activation mechanisms by different LDL apheresis systems
Extracorporeal removal of low-density lipoprotein (LDL) cholesterol by means of selective LDL apheresis is indicated in otherwise uncontrolled familial hypercholesterolemia. During blood–biomaterial interaction other constituents than the LDL particles are affected, including the complement system. We set up an ex vivo model in which human whole blood was passed through an LDL apheresis system with one of three different apheresis columns: whole blood adsorption, plasma adsorption and plasma filtration. The concentrations of complement activation products revealed distinctly different patterns of activation and adsorption by the different systems. Evaluated as the final common terminal complement complex (TCC) the whole blood system was inert, in contrast to the plasma systems, which generated substantial and equal amounts of TCC. Initial classical pathway activation was revealed equally for both plasma systems as increases in the C1rs–C1inh complex and C4d. Alternative pathway activation (Bb) was most pronounced for the plasma adsorption system. Although the anaphylatoxins (C3a and C5a) were equally generated by the two plasma separation systems, they were efficiently adsorbed to the plasma adsorption column before the “outlet”, whereas they were left free in the plasma in the filtration system. Consequently, during blood–biomaterial interaction in LDL apheresis the complement system is modulated in different manners depending on the device composition.
Anders Hovland, Randolf Hardersen, Erik Waage Nielsen, Terje Enebakk, Dorte Christiansen, Judith Krey Ludviksen, Tom Eirik Mollnes, Knut Tore Lappegård
Available online 30 June 2018
Source:Computational Biology and Chemistry Author(s): Ikrormi Rungsung, Muthukumaran Rajagopalan, Amutha Ramaswamy LKB1 protein is involved in the regulation of cell polarity by phosphorylating the AMPK under energetic stress condi ... more
15 September 2018
Source:European Journal of Pharmaceutical Sciences, Volume 122 Author(s): Nebojsa Mihajlica, Christer Betsholtz, Margareta Hammarlund-Udenaes Pericytes are perivascular cells that play important roles in the regulation of the blood-brain barri ... more
15 September 2018
Source:European Journal of Pharmaceutical Sciences, Volume 122 Author(s): Adrienn Kazsoki, Attila Domján, Károly Süvegh, Romána Zelkó Papaverine hydrochloride loaded gels, films and electrospun fibers were prepared for buccal drug delivery w ... more
Chronic hepatitis B virus (HBV) can progress to cirrhosis and hepatocellular carcinoma (HCC). A study describes a new HBV assay that offers advantages over currently used methods because it has the capability to detect closed circular DNA (cccDNA) in serum, single cells, and preserved tissu ... more
Angiogenesis, the formation of new blood vessels, is essential for tumor growth. A new study describes a vascular stabilization biomarker that can visualize blood vessel activity, thus optimizing the timing of anticancer therapies including anti-angiogenics.
Combination therapy using angiog ... more
Microscopic crystals could soon be zipping drugs around your body, taking them to diseased organs. In the past, this was thought to be impossible - the crystals, which have special magnetic properties, were so small that scientists could not control their movement. But now a team of Chinese ... more