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Differentiated human airway organoids to assess infectivity of emerging influenza virus [Microbiology]

Influenza virus infection represents a major threat to public health worldwide. There is no biologically relevant, reproducible, and readily available in vitro model for predicting the infectivity of influenza viruses in humans. Based on the long-term expanding 3D human airway organoids, we developed proximal differentiation and further established a 2D monolayer culture of airway organoids. The resultant 3D and 2D proximal differentiated airway organoids can morphologically and functionally simulate human airway epithelium and as a proof of concept can discriminate human-infective influenza viruses from poorly human-infective viruses. Thus, the proximal differentiated airway organoids can be utilized to predict the infectivity of influenza viruses and, more broadly, provide a universal platform for studying the biology and pathology of the human airway.

Authors:   Jie Zhou; Cun Li; Norman Sachs; Man Chun Chiu; Bosco Ho-Yin Wong; Hin Chu; Vincent Kwok-Man Poon; Dong Wang; Xiaoyu Zhao; Lei Wen; Wenjun Song; Shuofeng Yuan; Kenneth Kak-Yuen Wong; Jasper Fuk-Woo Chan; Kelvin Kai-Wang To; Honglin Chen; Hans Clevers; Kwok-Yung Yuen
Journal:   Proceedings of the National Academy of Sciences current issue
Volume:   115
edition:   26
Year:   2018
Pages:   6822
DOI:   10.1073/pnas.1806308115
Publication date:   26-Jun-2018
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