Marine Drugs, Vol. 16, Pages 207: The Holo-Transcriptome of the Zoantharian Protopalythoa variabilis (Cnidaria: Anthozoa): A Plentiful Source of Enzymes for Potential Application in Green Chemistry, Industrial and Pharmaceutical Biotechnology
Marine Drugs doi: 10.3390/md16060207
Authors:
Jean-Étienne R. L. Morlighem
Chen Huang
Qiwen Liao
Paula Braga Gomes
Carlos Daniel Pérez
Álvaro Rossan de Brandão Prieto-da-Silva
Simon Ming-Yuen Lee
Gandhi Rádis-Baptista
Marine invertebrates, such as sponges, tunicates and cnidarians (zoantharians and scleractinian corals), form functional assemblages, known as holobionts, with numerous microbes. This type of species-specific symbiotic association can be a repository of myriad valuable low molecular weight organic compounds, bioactive peptides and enzymes. The zoantharian Protopalythoa variabilis (Cnidaria: Anthozoa) is one such example of a marine holobiont that inhabits the coastal reefs of the tropical Atlantic coast and is an interesting source of secondary metabolites and biologically active polypeptides. In the present study, we analyzed the entire holo-transcriptome of P. variabilis, looking for enzyme precursors expressed in the zoantharian-microbiota assemblage that are potentially useful as industrial biocatalysts and biopharmaceuticals. In addition to hundreds of predicted enzymes that fit into the classes of hydrolases, oxidoreductases and transferases that were found, novel enzyme precursors with multiple activities in single structures and enzymes with incomplete Enzyme Commission numbers were revealed. Our results indicated the predictive expression of thirteen multifunctional enzymes and 694 enzyme sequences with partially characterized activities, distributed in 23 sub-subclasses. These predicted enzyme structures and activities can prospectively be harnessed for applications in diverse areas of industrial and pharmaceutical biotechnology.
Authors: |
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R. L. Morlighem, Jean-Étienne ; Huang, Chen ; Liao, Qiwen ; Braga Gomes, Paula ; Daniel Pérez, Carlos ; de Brandão Prieto-da-Silva, Álvaro Rossan; Ming-Yuen Lee, Simon ; Rádis-Baptista, Gandhi |
Journal: |
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Marine Drugs
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Volume: |
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16 |
edition: |
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6 |
Year: |
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2018 |
Pages: |
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207 |
DOI: |
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10.3390/md16060207
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Publication date: |
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13-Jun-2018 |