My watch list
my.bionity.com  
Login  

Reply to Queimado et al.: E-cigarettes induce DNA damage and inhibit DNA repair in mice and human cells [Biological Sciences]

We believe that Queimado et al. (1) may have inadvertently missed several key details presented in our recent PNAS publication (2). First, for the determination of nicotine effects on DNA adduct formation and DNA repair activity, the nicotine concentrations used for 1-h treatments of human lung and bladder epithelial cells were based on cytotoxicity (killing <20%). For lung cells, the concentrations used were 0, 100, and 200 μM. For urothelial cells, the concentrations used were 0, 1, and 2.5 μM (163–410 ng/mL), which are similar to the nicotine level generated by 100 puffs of an E-cigarette (2 … [↵][1]1Email: moon-shong.tang{at}nyumc.org. [1]: #xref-corresp-1-1

Authors:   Moon-shong Tang
Journal:   Proceedings of the National Academy of Sciences current issue
Volume:   115
edition:   24
Year:   2018
Pages:   E5439
DOI:   10.1073/pnas.1807971115
Publication date:   12-Jun-2018
Facts, background information, dossiers
More about Proceedings of the National Academy of Sciences
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE