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Anti-haemostatic compounds from the vampire snail Cumia reticulata: Molecular cloning and in-silico structure-function analysis

Publication date:

August 2018


Source:Computational Biology and Chemistry, Volume 75

Author(s): Maria Vittoria Modica, Jonathan Reinoso Sánchez, Andrea Pasquadibisceglie, Marco Oliverio, Paolo Mariottini, Manuela Cervelli

Blood-feeding animals are known for their ability to produce bioactive compounds to impair haemostasis and suppress pain perception in the host. These compounds are extremely appealing for pharmacological development since they are generally very effective and specific for their molecular target. A preliminary RNA-Seq based characterization of the secretion from salivary and mid-oesophageal tissues of the vampire snail Cumia reticulata, revealed a complex mixture of feeding-related transcripts with potential anaesthetic and anticoagulant action. Based on the cloned full-length mRNAs, it was possible to verify the sequence of five genes encoding haematophagy-related products. The in silico modelled three-dimensional structure of each translational product was analysed to gain information on their potential biochemical activity. We have hereby validated and further investigated the assembled transcripts presumably involved in the antihaemostatic action, to improve our comprehensive understanding of this subset of the feeding secretion. The studied proteins included both inhibitors of primary haemostasis such as the vWFA domain-containing proteins, and compounds targeting different steps of the coagulation cascade, as e.g. the Turripeptide-like/protease inhibitor, the TFPI-like multiple Kunitz-type protease inhibitors, the Meprin-like metalloproteases and the Astacin/ShKT-like domain-containing proteins. All these molecules showed promising potential for pharmacological development.
Graphical abstract




Authors:   Author(s): Maria Vittoria Modica, Jonathan Reinoso Sánchez, Andrea Pasquadibisceglie, Marco Oliverio, Paolo Mariottini, Manuela Cervelli
Journal:   Computational Biology and Chemistry
Volume:   75
Year:   2018
Pages:   168
DOI:   10.1016/j.compbiolchem.2018.05.014
Publication date:   27-May-2018
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