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Laterally confined growth of cells induces nuclear reprogramming in the absence of exogenous biochemical factors [Applied Physical Sciences]

In this study, we demonstrate a platform for reprogramming somatic cells with high efficiency in the absence of exogenous reprogramming factors. Sustained laterally confined growth of cells on micropatterned substrates results in sequential changes to the nucleus and chromatin with each cell division, leading to the progressive erasure of lineage specific characteristics and incorporation of pluripotency. After 10 days of confined growth, the cells exhibit stemness and have multilineage differentiation potential. Our observation highlights a previously unknown role of mechanical constraints in nuclear reprogramming. Our method provides a unique approach to greatly improve stem cell technologies for developing patient specific disease models and regenerative medicine.

Authors:   Bibhas Roy; Saradha Venkatachalapathy; Prasuna Ratna; Yejun Wang; Doorgesh Sharma Jokhun; Mallika Nagarajan; G. V. Shivashankar
Journal:   Proceedings of the National Academy of Sciences current issue
Volume:   115
edition:   21
Year:   2018
Pages:   E4741
DOI:   10.1073/pnas.1714770115
Publication date:   22-May-2018
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