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The Basic Polymerase Chain Reaction (PCR)

This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR).

Authors:   Michael R. Green; Joseph Sambrook
Journal:   CSH Protocols
Volume:   2018
edition:   5
Year:   2018
DOI:   10.1101/pdb.prot095117
Publication date:   01-May-2018
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