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Fluorescence lifetime and UV‐Vis spectroscopy to evaluate the interactions between quercetin and its yeast microcapsule

Quercetin is a fragile bioactive compound. Several works have tried to preserve it by encapsulation but the form of encapsulation (mono‐ or supra‐molecular structure, tautomeric form), though important for stability and bioavailability, remains unknown. The present work aims at developing a fluorescence lifetime technique to evaluate the structure of quercetin during encapsulation in a vector capsule that has already proven efficiency, yeast cells. Molecular stabilization was observed during a four‐month storage period. The time‐correlated single‐photon counting (TCSPC) technique was used to evaluate the interaction between quercetin molecules and the yeast capsule. The various tautomeric forms, as identified by UV‐Vis spectroscopy, resulted in various lifetimes in TCSPC, although they varied also with the buffer environment. Quercetin in buffer exhibited a three‐to‐four longer long time after 24 h (changing from 6‐7 to 18‐23 ns), suggesting an aggregation of molecules. In yeast microcapsules, the long‐time population exhibited a longer lifetime (around 27 ns) from the beginning and concerned about 20% of molecules compared to dispersed quercetin. This shows that lifetime analysis can show the monomolecular instability of quercetin in buffer and the presence of interactions between quercetin molecules and their microcapsules.

Authors:   Bao‐Ngoc Pham‐Hoang, Pascale Winckler, Yves Waché
Journal:   Biotechnology Journal
Year:   2017
Pages:   n/a
DOI:   10.1002/biot.201700389
Publication date:   09-Sep-2017
Facts, background information, dossiers
  • microcapsules
  • environment
  • encapsulation
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