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The microbiological standard method for the detection of Salmonella spp. is time‐consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open‐formula real‐time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in‐house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I ‐ Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest Ct values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check® Salmonella II kit, Bio‐Rad, USA).Practical applications
The whole procedure of real‐time PCR methods in this study was approximately 20 hr, in contrast to 4–5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real‐time PCR method also provides a high level of sensitivity and specificity with a low risk of cross‐contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.
Abstract The single radial immunodiffusion assay has been the accepted method for determining the potency of inactivated influenza vaccines since 1978. The world‐wide adoption of this assay for vaccine standardisation was facilitated through collaborative studies that demonstrated a high ... more
Abstract Background Whether morbidity from the 1918‐19 influenza pandemic discriminated by socioeconomic status has remained a subject of debate for 100 years. In lack of data to study this issue recent literature have hypothesized that morbidity was “socially neutral”. Objectives ... more
Smart skin that can respond to external stimuli could have important applications in medicine and robotics. Using only items found in a typical household, researchers have created multi-sensor artificial skin that's capable of sensing pressure, temperature, humidity, proximity, pH, and air ... more
Some doctors may recommend that patients with the flu take acetaminophen, or paracetemol, to relieve their symptoms; however, a new randomized clinical trial found no benefits to the over-the-counter medication in terms of fighting the influenza virus or reducing patients' temperature or ot ... more
A new study that investigated the potential of certain psychological traits for predisposing heterosexuals to have negative attitudes towards homosexual people found that psychoticism - which is present in severe psychopathological conditions but may also contribute to less severe states of ... more