My watch list  

Locoregional Effects of Microbiota in a Preclinical Model of Colon Carcinogenesis

Inflammation and microbiota are critical components of intestinal tumorigenesis. To dissect how the microbiota contributes to tumor distribution, we generated germ-free (GF) ApcMin/+ and ApcMin/+ ; Il10−/− mice and exposed them to specific-pathogen-free (SPF) or colorectal cancer-associated bacteria. We found that colon tumorigenesis significantly correlated with inflammation in SPF-housed ApcMin/+ ; Il10−/− , but not in ApcMin/+ mice. In contrast, small intestinal neoplasia development significantly correlated with age in both ApcMin/+ ; Il10−/− and ApcMin/+ mice. GF ApcMin/+ ; Il10−/− mice conventionalized by an SPF microbiota had significantly more colon tumors compared with GF mice. Gnotobiotic studies revealed that while Fusobacterium nucleatum clinical isolates with FadA and Fap2 adhesins failed to induce inflammation and tumorigenesis, pks + Escherichia coli promoted tumorigenesis in the ApcMin/+ ; Il10−/− model in a colibactin-dependent manner, suggesting colibactin is a driver of carcinogenesis. Our results suggest a distinct etiology of cancers in different locations of the gut, where colon cancer is primarily driven by inflammation and the microbiome, while age is a driving force for small intestine cancer. Cancer Res; 77(10); 2620–32. ©2017 AACR .

Authors:   Sarah Tomkovich; Ye Yang; Kathryn Winglee; Josee Gauthier; Marcus Mühlbauer; Xiaolun Sun; Mansour Mohamadzadeh; Xiuli Liu; Patricia Martin; Gary P. Wang; Eric Oswald; Anthony A. Fodor; Christian Jobin
Journal:   Cancer Research
Volume:   77
edition:   10
Year:   2017
Pages:   2620
DOI:   10.1158/0008-5472.CAN-16-3472
Publication date:   15-May-2017
Facts, background information, dossiers
More about AACR Publications
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE