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Macro and 3D Imaging with Cellular Resolution Using Light Sheet Fluorescence Microscopy

UltraMicroscope II Delivers High Speed, Optical Sectioning to Complement Macro in Vivo Techniques

LaVision BioTec’s UltraMicroscope II delivers light sheet fluorescence microscopy (LSFM) and scanning plane illumination microscopy (SPIM) for high speed 3D whole brain imaging

Mouse embryo E12 tag1 ChAT; courtesy of Chloé Dominici and Alain Chédotal, Institut de la Vision, Paris, France

Mouse hippocampus, Thy-1 GFP, courtesy of Bianca Schmid, Max-Planck-Institute for Psychiatry, Munich, Germany

Drosophila melanogaster (larvae) GFP, Autofluorescence

Mouse Embryo E10.5 Vascular System Courtesy of: R. Hägerling, C. Pollmann, F. Kiefer Max-Planck-Institute for Molecular Biomedicine, Münster

UltraMicroscope II

The LaVision BioTec UltraMicroscope II light sheet microscope combines macro-imaging capabilities with cellular resolution and allows 3D imaging of cleared samples in aqueous and organic buffers. The UltraMicroscope provides the perfect complementary microscopic technique to macro in vivo techniques like Bioluminescence Imaging (BLI), Single Photon Emission Computed Tomography (SPECT) and Magnetic Resonance Imaging (MRI).

The optical principle of the UltraMicroscope is known as SPIM (Selective plane illumination microscopy) or LSFM (Light Sheet Fluorescence Microscopy). It allows high resolution, excellent optical sectioning and high speed imaging. The UltraMicroscope II is the only system that connects these features with macroscopic sample dimensions up to 1 cm³ or larger.

Key bullets:

  • whole brain, organ and embryo imaging at cellular resolution

  • image in aqueous buffers and in organic solvents

  • sample size over 1 cm to few µm

  • 3D high speed imaging without bleaching

  • clearings: Scale, SeeDB, CLARITY, THF, DBE, 3DISCO, iDISCO, CUBIC, etc.

Application areas:

  • neuroscience
  • immunology
  • oncology
  • developmental biology
  • cancer research
  • physiology

Researchers who need artefact-free data from an overview to a specific region of interest with cellular resolution apply ultramicroscopy in their work. For example, neuroscientists focusing on the regeneration potential of neurons and the axonal pathfinding use this system as do oncologists verifying the efficiency of neovascularization inhibitors. In the field of immunology, lymph nodes and the developmental steps of entire lymphatic system are analyzed. The different developmental stages of animal models can be imaged for phenotyping or characterization of pathologies. Image acquisition made in vivo as well as the imaging of samples prepared by any clearing procedure are possible. Tissue with endogenous fluorescent proteins like GFP or stained with labelled antibodies can be analyzed quickly and easily with this setup. Clearing procedures like 3DISCO and CUBIC have been developed with the UltraMicroscope.

Complementary technology from LaVision BioTec:

TriM Scope II

Two-photon excitation microscopy is a fluorescence imaging technique that allows imaging of living tissue up to about one millimeter in depth. Being a special variant of the multiphoton fluorescence microscope, it uses red-shifted excitation light which can also excite fluorescent dyes.

Self-aligning 2-Photon Microscope

  • optimized for deep penetration in the single beam mode, with high efficiency collection optics, pre-chirp compensation, optimized beam diameter and Ultra-sensitive PMT's
  • optimized scan speed.  For samples that require high frame rates, use the 64 beam scan mode or resonant scanner for frame rates of 10-100 per second
  • OPO for red probes and proteins. Deep penetration for red-dyes
  • simultaneous OPO and Ti:Sa scanning

Potential measurements

  • fast intravital imaging with multiplexed beams and resonant scanner   
  • FRAP, photo-activation / -stimulation, uncaging, optogenetics, and line scanning  
  • SHG and THG imaging with OPO and Ti:Sa laser
  • integrated FLIM imaging with LaVision BioTec’s FLIM detector
  • ultrafast 64 beam imaging with up to 100 frames/s @ 2560x2160 pixel

Benefits for 2-photon microscopy

  • excitation of red dyes
  • reduced phototoxicity and bleaching
  • high imaging depth with 1100 - 1600 nm @ 200 fs
  • optimized SHG and THG excitation and detection

Visit the LaVision BioTec image gallery to see how these exciting microscopies may be applied. The images are stunning. Also, check out the bibliography lists and see the work of leading researchers worldwide.

Request information now or download our brochure.

More about LaVision BioTec
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    LaVision BioTec GmbH

    LaVision BioTec GmbH – we connect biotechnology and optics. The span of the technologies of this young company from Bielefeld reaches from intravital microscopy to fluorescent imagers for microarrays. The most prominent product is the TriMScope, a unique 2-photon microscope used to visualiz ... more

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