Ultra-Deep Microbiome Prep – Enrichment and Extraction of Microbial DNA from Tissue and Body Fluids

Depletion of non-target host DNA for ultra-deep microbiome sequencing

Sensitive characterisation of microbial communities

The Ultra-Deep Microbiome Prep kits are the perfect tool for the selective enrichment and extraction of bacterial and fungal DNA from a variety of tissue and body fluids. The kit combines protocols for the depletion of human/animal DNA (MolYsis™ technology) and extraction of microbial DNA.

Tissue samples: among others, heart valves, biopsies, brain, abscesses of brain, lymph nodes, liver, lung, skin, gut, FFPE tissue.

Body liquids: among others, blood, CSF, BAL, synovial fluid, pleural fluid, ascites fluid

The Ultra-Deep Microbiome Prep kits allow for significantly deeper sequencing compared to conventional DNA isolation. The benefits of the kits are:

  • Depletion of DNA from the host: allows the enrichment of intact bacteria and fungi from samples and the isolation of pure microbial DNA suitable for amplification.
  • Contamination-free microbial DNA isolation: Kits include all reagents, buffers, spin columns and plastics required for the sensitive and contamination-free microbial DNA isolation from samples.

DNA enriched by the Ultra-Deep Microbiome Prep kits is compatible to commercially available NGS library and sequencing kits.

Product Facts:

  • Sensitivity: Reduced background level by depletion of host DNA for ultra-deep analysis of microbiomes
  • Purity: Consumables, buffers and reagents are quality controlled for the absence of contaminating microbial DNA
  • Extraction validated for a broad-range of Gram-positive and Gram-negative bacteria and fungi
  • For a variety of tissue and liquid specimens
  • Small to large volume protocols: 0.2 up to 10ml samples

Molzym – Innovative Products for Molecular Microbiology

Next Generation Sequencing (NGS) allows analysing polymicrobial infections and complex microbial communities independent of culture. High background of host DNA and reagent-borne contaminations limit the deeper analyses.

The proprietary enrichment technology (MolYsis™) of Molzym offers an easy solution for the isolation of microbial DNA from intact organisms. With diverse possible specimens from humans and animals, the Ultra-Deep Microbiome Prep kits are suitable for many applications.

Prepare your microbial DNA without contaminations by using ultra-clean, DNA-free reagents. This further enhances the sensitivity and accuracy of microbiome analyses.

For deep microbial sequencing Molzym offers a master mix for the 16S Amplicon PCR as part of the library preparation for 16S metagenomics sequencing with Illumina® MiSeq system.

For detailed information, please contact the Molzym support team.

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