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Ultra fast Absorbance Spectrometer

In Less Than a Second per Well: BMG LABTECH's FLUOstar Omega

The FLUOstar Omega microplate reader with Tandem Technology uses an ultra-fast UV/Vis spectrometer for flexibility in absorbance and high performance filters for detection in fluorescence

The FLUOstar Omega combines the flexibility and sensitivity of a classic UV/Vis-spectrophotometer with the speed and sample capacity of a microplate reader. Capture a full spectrum from 220-1000 nm in less than 1 second per well.

ELISA assay correction – 17% increase in dynamic range when measured at 430 nm with the spectrometer vs. normal filter measurement at 450 nm.

Two precise onboard injectors with an exceptional low dead volume allow simultaneous reagent injection and assay monitoring.

Well scanning in a 96 well microplate using a 10x10 matrix of data points. A 3D representation of a well shows two areas where cells are localized.

The FLUOstar Omega is a multi-mode microplate reader featuring BMG LABTECH's proprietary Tandem Technology. It uses an ultra-fast CCD-based spectrometer for UV/Vis absorbance and ultra-sensitive filters for detection in other modes. Unique to BMG LABTECH, the UV/Vis spectrometer can capture a full spectrum (220 to 1000 nm) at a 1 nm resolution in <1 sec/well. This technology allows for absorbance assays not possible on other microplate readers.

Other Features Include:

  • Up to 2 on-board reagent injectors
  • Top and bottom reading
  • Automatic Gain Adjustment
  • Stacker and robot compatible via DDE or ActiveX interface
  • Incubation up to 65°C
  • Variable shaking
  • Compatible with 6- to 1536-well microplates

Detection Modes Include:

  • UV/Vis Absorbance
  • Fluorescence Intensity, including FRET
  • Time-Resolved Fluorescence – including DELFIA®
  • TR-FRET – including HTRF® and LanthaScreen™
  • AlphaScreen® / AlphaLISA®
  • High-Performance Luminescence (flash and glow) - including BRET

Advanced Assay Technology Package for the FLUOstar Omega
A specialized optical system has been designed to provide Omega series with advanced detection modes that rival any HTS microplate reader, AlphaScreen/ AlphaLISA and TRF/TR-FRET. Experience fantastic AlphaScreen / AlphaLISA performance on a mid-range multi-mode microplate reader. Furthermore, the Advanced Assay Technology Package with a TRF assay specific dichroic mirror, the high intensity xenon flash lamp, and the adjustable gain, allow the Omega to outperform any microplate reader in the mid-range market.

The FLUOstar Omega Can Perform All of the Leading Microplate Reader Applications Including:

  • Protein and nucleic acid quantitation (Bradford, Lowry, DNA, RNA, 260/280)
  • Cell toxicity, proliferation, migration, and viability
  • Immunoassays, ELISA
  • Ca2+ measurements (e.g. Ca2+ flux with Fura-2, Indo-1, FLUO-3/4)
  • Enzyme activity
  • Luciferase, DLR®dual luciferase, BRET with sequential dual emission detection
  • ORAC and ABEL antioxidant assays
  • Fluorescent proteins, including GFP, mCherry, CFP/YFP FRET
  • TR-FRET, HTRF, LanthaScreen®, DELFIA, Transcreener®
  • Label-free SoPRano™ Gold Nanoparticle assays

Cell-based Assays Benefit from ACU Gas Control Features
The optional Atmospheric Control Unit (ACU) offers exceptional O2 and CO2 regulation. The microprocessor controlled ACU fully regulates O2 and CO2 within the microplate reader allowing you to select the concentrations that match your required physiological conditions. The ACU adjusts the two gases independently, ensuring that any combination can be easily programmed, eliminating the need for premixed gases. In combination with extended temperature control up to 65°C and unrivalled shaking options (orbital, linear, double orbital, variable speed), the ACU provides a true solution for all cell-based assays. The ACU provides a solution to all atmosphere sensitive assays, including:

  • Mammalian and Stem Cell Assays
  • Hypoxia
  • Cell Viability
  • Cell Growth
  • Migration Studies
  • Invasion Assays
  • Intracellular pH
  • Angiogenesis
  • Viral Uptake
  • Bacterial Studies
  • Ischemia / Reperfusion and more

Upgradable to POLARstar Omega
The Omega microplate reader series has a modular design concept. This enables the FLUOstar Omega to be fully upgraded to the powerful POLARstar Omega, which can additionally perform Simultaneous Dual Emission and fluorescence polarization detection.

Request information now or download our brochure.

We are happy to answer your questions by phone at +49 (0) 781 / 956396-53

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