Since it was developed over 100 years ago, cell cultivation has become an indispensable tool in research and quality assurance. To obtain transferable results, the cells must be cultivated under conditions that are as similar to reality as possible.
Cell culture inserts allow researchers to imitate the natural cellular environment and open up unique experimental opportunities compared with the two-dimensional method on the bottom of culture vessels. With inserts, communication between different cell types can be analyzed by co-cultivation or studying the transcellular transport of substances. In addition, cell culture inserts can be used to investigate the reaction of cells to various substances, e.g. with chemotaxis, drug transport and migration assays. Last but not least, 3D epithelial models cultivated in cell culture inserts are used to estimate the corrosive or irritating properties of chemicals on human skin.
Earlier cell culture insert systems had several shortcomings. Prior to starting the test, users had to decide whether they wanted to utilize the cell culture inserts in Petri dishes or multiwell plates in a standing or hanging position. Both variants have their own advantages and disadvantages. If it became evident during initial tests that the other insert system would be more suitable, the entire setup would have to be changed, which is both time-consuming and costly. This meant changing the cultivation surfaces and thus an alteration of test parameters.
The BRAND® Insert 2in1 cell culture insert eliminates this system drawback. As the first insert that can be used in both a hanging and standing position without any additional accessories, it gives users complete flexibility. Equipped with a catch and three stands, the inserts fit exactly in 6-, 12-, and 24-well plates. This design consistently allows adequate room for a clean, low-contamination change of media. If the inserts are placed in a standing or hanging position, the stands and catch ensure that the membrane is placed securely over the bottom of the cell culture vessel with a parallel orientation.
Many cell lines thrive particularly well thanks to the cellGradeTM plus surface – without any additional coatings. Consistent cell growth ensures reproducible results for a wide variety of analyses.
The cell culture inserts are available individually packaged or in multi-packs for practical use and conservation of resources. Depending on the size, the multi-packs contain six, twelve, or 24 inserts,
which can be removed in groups of three as needed, while the rest of the inserts remain in the sterile packaging.
The biocompatibility of the PC and PET membranes is also certified in accordance with USP Class VI. This keeps interaction between the plastic and the cells as low as possible, so as to obtain valid results.