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Raman Imaging Purpose-Built for Life Sciences:
Label-Free Characterization with Ease and Efficiency

Every advantage of WITec’s unique 3D Raman technology is now available in an inverted microscope

The alpha300 Ri inverted Raman imaging system offers many advantages for Life Sciences applications.

Confocal 3D Raman image of the flesh of a banana. Gre starch grains, red: cell wall components

Correlative Raman fluorescence microscopy. Left: cell nuclei stained with DAPI. Right: their Raman spectra can be used to identify nucleoli (green) and ER (red) without staining. Here the Raman image and the fluorescence image were superimposed.

Experience Raman imaging from a new angle: The inverted beam path enables easy and straightforward operation of the microscope and positioning of the sample when acquiring confocal Raman images. Research applications in life sciences, biomedicine and pharmaceutics can benefit in particular from the new setup as their samples are often examined from below.

The geometry of the inverted alpha300 Ri microscope makes work easier for the user while increasing efficiency and throughput in the laboratory. The examination of samples in a liquid environment, e.g. cell cultures, can now be carried out much more effectively. Standardized sample containers can be secured and switched quickly and reproducibly, which is especially advantageous for routine work. Accessories or special chambers and incubators can be easily mounted on the motorized sample stage. Materials science examinations also benefit from the inverted beam path because even large, tall samples will fit between the stage and condenser.

Due to the modular design of the WITec alpha300 microscope platform, label-free chemical imaging using Raman spectroscopy can also be precisely correlated with established fluorescence microscopy. In addition, the instrument can be enhanced with other microscopy techniques such as phase contrast and differential interference contrast (DIC) to provide even more comprehensive image data.

Raman microscopy can be used to visualize the chemical composition of samples. It is non-destructive, requires no dyes and only minimal sample preparation, if any. WITec's Raman microscope technology offers unmatched performance in speed, sensitivity and resolution for breakthrough research.

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