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29 Aktuelle Fachpublikationen zum Thema Hefen

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Viruses, Vol. 10, Pages 281: Yeast Derived LysA2 Can Control Bacterial Contamination in Ethanol Fermentation

24.05.2018 | Kim, Jun-Seob ; Daum, M. Angela; Jin, Yong-Su ; Miller, Michael J., Viruses, 2018

Viruses, Vol. 10, Pages 281: Yeast Derived LysA2 Can Control Bacterial Contamination in Ethanol Fermentation Viruses doi: 10.3390/v10060281 Authors: Jun-Seob Kim M. Angela Daum Yong-Su Jin Michael J. Miller Contamination of fuel-ethanol fermentations continues to be a ...

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Viruses, Vol. 10, Pages 281: Yeast Derived LysA2 Can Control Bacterial Contamination in Ethanol Fermentation

24.05.2018 | Kim, Jun-Seob ; Daum, M. Angela; Jin, Yong-Su ; Miller, Michael J., Viruses, 2018

Viruses, Vol. 10, Pages 281: Yeast Derived LysA2 Can Control Bacterial Contamination in Ethanol Fermentation Viruses doi: 10.3390/v10060281 Authors: Jun-Seob Kim M. Angela Daum Yong-Su Jin Michael J. Miller Contamination of fuel-ethanol fermentations continues to be a ...

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Marine Drugs, Vol. 16, Pages 158: High-Level Expression of a Thermally Stable Alginate Lyase Using Pichia pastoris, Characterization and Application in Producing Brown Alginate Oligosaccharide

11.05.2018 | Li, Haifeng ; Wang, Shuling ; Zhang, Yunyi ; Chen, Liehuan, Marine Drugs, 2018

Marine Drugs, Vol. 16, Pages 158: High-Level Expression of a Thermally Stable Alginate Lyase Using Pichia pastoris, Characterization and Application in Producing Brown Alginate Oligosaccharide Marine Drugs doi: 10.3390/md16050158 Authors: Haifeng Li Shuling Wang Yunyi Zhang Liehuan ...

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Design, expression, and characterization of the hybrid antimicrobial peptide T‐catesbeianin‐1 based on FyuA

11.01.2018 | Huihui Xu, Kunyuan Tie, Yang Zhang, Xin Feng, Yuan Cao, Wenyu Han, Journal of Peptide Science, 2018

The overuse of antibiotics has resulted in the emergence of antibiotic‐resistant bacteria, which presents an urgent need for new antimicrobial agents. At present, antimicrobial peptides have attracted a great deal of attention from researchers. However, antimicrobial peptides often affect a ...

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An environment‐friendly approach to isolate and purify glucan from spent cells of recombinant Pichia pastoris and the bioactivity characterization of the purified glucan

29.12.2017 | Yan Xing, Chaonan Chen, Wenlong Sun, Bowei Zhang, Yuanbin Sang, Zhilong Xiu, Yuesheng Dong, Engineering in Life Sciences, 2017

Abstract While the methylotrophic yeast Pichia pastoris enables the industrial‐scale biosynthesis of many recombinant products, large amount of nutrient‐rich biomass emerged along this process. Polysaccharides, especially glucans that are abundant in the cell wall of P. pastoris, are yet to be ...

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Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

27.12.2017 | Thomas Vogl, Lukas Sturmberger, Pia C. Fauland, Patrick Hyden, Jasmin Elgin Fischer, Christian Schmid, Gerhard G. Th ..., Biotechnology and Bioengineering, 2017

Abstract Carbon source regulated promoters are well‐studied standard tools for controlling gene expression. Acquiring control over the natural regulation of promoters is important for metabolic engineering and synthetic biology applications. In the commonly used protein production host ...

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Development of a high‐throughput microscale cell disruption platform for Pichia pastoris in rapid bioprocess design

08.09.2017 | Benjamin A. F. Bláha, Stephen A. Morris, Olotu W. Ogonah, Vincenzo Crescente, William Rosenberg, Sophie Maucourant, ..., Biotechnology Progress, 2017

Abstract The time and cost benefits of miniaturized fermentation platforms can only be gained by employing complementary techniques facilitating high‐throughput at small sample volumes. Microbial cell disruption is a major bottleneck in experimental throughput and is often restricted to ...

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Development of a general defined medium for Pichia pastoris

02.09.2017 | Catherine Bartlett Matthews, Angel Kuo, Kerry Routenberg Love, J. Christopher Love, Biotechnology and Bioengineering, 2017

Abstract Pichia pastoris is widely used as a host for recombinant protein production. More than 500 proteins have been expressed in the organism at a variety of cultivation scales, from small shake flasks to large bioreactors. Large‐scale fermentation strategies typically employ ...

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Advanced monitoring and control of pharmaceutical production processes with Pichia pastoris by using Raman spectroscopy and multivariate calibration methods

01.09.2017 | Jan‐Patrick Voss, Nina E. Mittelheuser, Roman Lemke, Reiner Luttmann, Engineering in Life Sciences, 2017

This contribution includes an investigation of the applicability of Raman spectroscopy as a PAT analyzer in cyclic production processes of a potential Malaria vaccine with Pichia pastoris. In a feasibility study, Partial Least Squares Regression (PLSR) models were created off‐line for cell ...

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A new type of gene‐disruption cassette with a rescue gene for Pichia pastoris

25.08.2017 | Tatsuro Shibui, Hiroyoshi Hara, Biotechnology Progress, 2017

Abstract Pichia pastoris has been used for the production of many recombinant proteins, and many useful mutant strains have been created. However, the efficiency of mutant isolation by gene‐targeting is usually low and the procedure is difficult for those inexperienced in yeast genetics. In ...

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