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3.415 Aktuelle Fachpublikationen in Journal of Proteome Research

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Quantification of Total Vitamin-D-Binding Protein and the Glycosylated Isoforms by Liquid Chromatography–Isotope Dilution Mass Spectrometry

16.10.2017 | Lisa E. Kilpatrick; Karen W. Phinney, Journal of Proteome Research, 2017

Vitamin-D-binding protein (VDBP), a transporter of 25-hydroxyvitamin D metabolites, has three common isoforms. The relationship of the isoforms and their glycosylation state with various diseases has been under recent examination. In this work, liquid chromatography coupled to isotope dilution ...

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Aminoquinoline Fluorescent Labels Obstruct Efficient Removal of N-Glycan Core α(1–6) Fucose by Bovine Kidney α-l-Fucosidase (BKF)

13.10.2017 | Róisín O’Flaherty; Aoife M. Harbison; Philip J. Hanley; Christopher H. Taron; Elisa Fadda; Pauline M. Rudd, Journal of Proteome Research, 2017

Here we report evidence that new aminoquinoline N-glycan fluorescent labels interfere with the release of core α(1–6) fucose from N-glycans by bovine kidney α-l-fucosidase (BKF). BKF is a commonly employed exoglycosidase for core α(1–6) fucose determination. Molecular simulations of the bound and ...

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Comparison and Evaluation of Clustering Algorithms for Tandem Mass Spectra

13.10.2017 | Vera Rieder; Karin U. Schork; Laura Kerschke; Bernhard Blank-Landeshammer; Albert Sickmann; Jörg Rahnenführer, Journal of Proteome Research, 2017

In proteomics, liquid chromatography–tandem mass spectrometry (LC–MS/MS) is established for identifying peptides and proteins. Duplicated spectra, that is, multiple spectra of the same peptide, occur both in single MS/MS runs and in large spectral libraries. Clustering tandem mass spectra is used ...

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Evaluation of FASP, SP3, and iST Protocols for Proteomic Sample Preparation in the Low Microgram Range

11.10.2017 | Malte Sielaff; Jörg Kuharev; Toszka Bohn; Jennifer Hahlbrock; Tobias Bopp; Stefan Tenzer; Ute Distler, Journal of Proteome Research, 2017

Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Here, we performed an independent comparison between single-pot solid-phase-enhanced sample preparation (SP3), filter-aided sample preparation (FASP), and a ...

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Discovery of Tamoxifen and N-Desmethyl Tamoxifen Protein Targets in MCF-7 Cells Using Large-Scale Protein Folding and Stability Measurements

11.10.2017 | Ryenne N. Ogburn; Lorrain Jin; He Meng; Michael C. Fitzgerald, Journal of Proteome Research, 2017

The proteins in an MCF-7 cell line were probed for tamoxifen (TAM) and n-desmethyl tamoxifen (NDT) induced stability changes using the Stability of Proteins from Rates of Oxidation (SPROX) technique in combination with two different quantitative proteomics strategies, including one based on SILAC ...

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Discovery of Tamoxifen and N-Desmethyl Tamoxifen Protein Targets in MCF-7 Cells Using Large-Scale Protein Folding and Stability Measurements

11.10.2017 | Ryenne N. Ogburn; Lorrain Jin; He Meng; Michael C. Fitzgerald, Journal of Proteome Research, 2017

The proteins in an MCF-7 cell line were probed for tamoxifen (TAM) and n-desmethyl tamoxifen (NDT) induced stability changes using the Stability of Proteins from Rates of Oxidation (SPROX) technique in combination with two different quantitative proteomics strategies, including one based on SILAC ...

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The Human Plasma Proteome Draft of 2017: Building on the Human Plasma PeptideAtlas from Mass Spectrometry and Complementary Assays

10.10.2017 | Jochen M. Schwenk; Gilbert S. Omenn; Zhi Sun; David S. Campbell; Mark S. Baker; Christopher M. Overall; Ruedi Aebers ..., Journal of Proteome Research, 2017

Human blood plasma provides a highly accessible window to the proteome of any individual in health and disease. Since its inception in 2002, the Human Proteome Organization’s Human Plasma Proteome Project (HPPP) has been promoting advances in the study and understanding of the full protein ...

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Analysis of O-Glycopeptides by Acetone Enrichment and Capillary Electrophoresis-Mass Spectrometry

10.10.2017 | Montserrat Mancera-Arteu; Estela Giménez; Fernando Benavente; José Barbosa; Victòria Sanz-Nebot, Journal of Proteome Research, 2017

Acetone precipitation was evaluated as a rapid, simple, low-cost, and efficient method for the selective purification of O-glycopeptides from enzymatic digests of glycoproteins. Ovalbumin (OVA), human and bovine α1-acid glycoprotein (hAGP and bAGP), human apolipoprotein C–III (APO-C3), and ...

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Multiplex and Label-Free Relative Quantification Approach for Studying Protein Abundance of Drug Metabolizing Enzymes in Human Liver Microsomes Using SWATH-MS

10.10.2017 | Rohitash Jamwal; Benjamin J. Barlock; Sravani Adusumalli; Ken Ogasawara; Brigitte L. Simons; Fatemeh Akhlaghi, Journal of Proteome Research, 2017

We describe a sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) based method for label-free, simultaneous, relative quantification of drug metabolism enzymes in human liver microsomes (HLM; n = 78). In-solution tryptic digestion was aided by a pressure ...

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Automatic Identification and Quantification of Extra-Well Fluorescence in Microarray Images

10.10.2017 | Robert Rivera; Jie Wang; Xiaobo Yu; Gokhan Demirkan; Marika Hopper; Xiaofang Bian; Tasnia Tahsin; D. Mitchell Magee; ..., Journal of Proteome Research, 2017

In recent studies involving NAPPA microarrays, extra-well fluorescence is used as a key measure for identifying disease biomarkers because there is evidence to support that it is better correlated with strong antibody responses than statistical analysis involving intraspot intensity. Because this ...

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